| EGb761对谷氨酸诱导海马神经元兴奋毒性损伤中p75NTR表达的影响 |
| |
| 引用本文： | 吴兰香,滕永真,李春莉,吴明军. EGb761对谷氨酸诱导海马神经元兴奋毒性损伤中p75NTR表达的影响[J]. 医学争鸣, 2009, 0(19): 1901-1904 |
| |
| 作者姓名： | 吴兰香 滕永真 李春莉 吴明军 |
| |
| 作者单位： | 重庆医科大学生命科学研究院; |
| |
| 基金项目： | 重庆医科大学校级科研课题(0200101097) |
| |
| 摘 要： | 目的：观察谷氨酸诱导海马神经元兴奋毒性损伤中神经营养因子受体p75（p75neurotrophin receptor,p75NTR）表达水平的变化,探讨银杏叶提取物（EGb761）抗兴奋毒性神经保护作用与p75NTR的相关性.方法：建立谷氨酸诱导的新生大鼠海马神经元兴奋毒性损伤模型,观察25200mg/L剂量下EGb761的神经保护作用;RT-PCR,Western Blot及细胞免疫荧光染色方法检测p75NTR的表达变化.结果：100mg/LEGb761预处理给药的神经保护效果最明显;谷氨酸刺激后,p75NTR在mRNA和蛋白质水平的表达较对照组明显增高（P〈0.01）;EGb761本身对p75NTR表达无影响,而EGb761预处理组p75NTR在mRNA和蛋白质水平的表达较谷氨酸组均显著降低（P〈0.05）.结论：p75NTR在谷氨酸诱导兴奋毒性神经损伤中起着重要作用,EGb761抗兴奋毒性神经保护作用可能与抑制p75NTR的表达有关.
|
| 关 键 词： | EGb761 谷氨酸 兴奋毒性 p75NTR 神经保护 |
Effect of EGb761 on expression of p75NTR in cultured hippocampal neurons after glutamate-induced excitotoxicity |
| |
| WU Lan-Xiang,TENG Yong-Zhen,LI Chun-Li,WU Ming-Jun. Effect of EGb761 on expression of p75NTR in cultured hippocampal neurons after glutamate-induced excitotoxicity[J]. Negative, 2009, 0(19): 1901-1904 |
| |
| Authors: | WU Lan-Xiang TENG Yong-Zhen LI Chun-Li WU Ming-Jun |
| |
| Affiliation: | WU Lan-Xiang,TENG Yong-Zhen,LI Chun-Li,WU Ming-Jun Department of Life Sciences,Chongqing Medical University,Chongqing 400036,China |
| |
| Abstract: | AIM:To observe the changes in the expression level of p75NTR(p75 neurotrophin receptor)in cultured hippocampal neurons after glutamate-induced excitotoxicity and to explore the correlation between the antiexcitotoxic effect of Ginkgo biloba extract(EGb761)and p75NTR.METHODS:Excitotoxicity was induced by glutamate in rat cultured hippocampal neurons and the neuroprotective effect of 25-200 mg/L EGb761 was observed.RT-PCR,Western blot and immunofluorescence staining were used to detect the changes in the expr... |
| |
| Keywords: | EGb761 glutamate excitotoxicity p75NTR neuroprotection |
| 本文献已被 CNKI 维普 等数据库收录！ |
|
