| 甲氧滴滴涕对小鼠着床期子宫钙结合蛋白D9k基因表达的影响 |
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| 引用本文： | 郅云国,马向东,陈必良. 甲氧滴滴涕对小鼠着床期子宫钙结合蛋白D9k基因表达的影响[J]. 医学争鸣, 2009, 0(14): 1306-1308 |
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| 作者姓名： | 郅云国 马向东 陈必良 |
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| 作者单位： | 第四军医大学西京医院妇产科; |
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| 摘 要： | 目的：研究甲氧滴滴涕（MXC）对小鼠着床期子宫钙结合蛋白D9k（Calbindin-D9k）基因表达的影响。方法：将真孕及假孕小鼠于孕1-3d（发现阴栓为孕0日）每日给予不同剂量的MXC（0，100，250，500mg／kg）灌胃，对照组（MXC 0mg／kg）只给芝麻油溶剂。采用RT-PCR检测各组孕鼠子宫Calbindin-D9k基因mRNA的表达；免疫组化染色和图像分析技术检测Calbindin-D9k蛋白表达。结果：①孕鼠经MXC染毒3d后，250，500mg／kg MXC组孕鼠子宫Calbindin-D9k mRNA的表达量与对照组相比有显著降低（P〈0.05），然而，100mg／kg MXC组Calbindin-D9k mRNA的表达与对照组相比虽有所减低，但无统计学意义（P〉0.05）；②Calbindin-D9k蛋白在孕5日主要表达在子宫内膜上皮细胞和腺上皮细胞。与mRNA的表达水平一致，250，500mg／kgMXC组孕鼠子宫Calbindin-D9k蛋白的表达量与对照组相比显著降低（P〈0.05），而100mg／kg MXC组Calbindin-D9k蛋白的表达与对照组相比无明显差异（P〉0.05）。结论：MXC可以降低小鼠着床期子宫Calbindin-D9k基因的表达，这可能是其干扰胚胎着床的一个毒性机制。
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| 关 键 词： | 甲氧氯 钙结合蛋白D9k 小鼠 子宫 |
Effects of methoxychlor on Calbindin-D9k gene expression in mouse uterus during implantation period |
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| ZHI Yun-Guo,MA Xiang-Dong,CHEN Bi-Liang. Effects of methoxychlor on Calbindin-D9k gene expression in mouse uterus during implantation period[J]. Negative, 2009, 0(14): 1306-1308 |
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| Authors: | ZHI Yun-Guo MA Xiang-Dong CHEN Bi-Liang |
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| Affiliation: | ZHI Yun-Guo,MA Xiang-Dong,CHEN Bi-Liang Department of Obstetrics and Gynecology,Xijing Hospital,Fourth Military Medical University,Xi'an 710033,China |
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| Abstract: | AIM: To investigate the effects of methoxychlor(MXC) on the expression of Calbindin-D9k gene in the mouse uterus during implantation period.METHODS: Pregnant and pseudopregnant mice were given daily by intragastric administration of MXC at different doses of 0(control),100,250 and 500 mg/kg from day 1 to day 3 of pregnancy(plug=Day 0),while the mice in the control group were given intragastrically sesame oil solvent during the same period.The expression of Calbindin-D9k mRNA was measured by RT-PCR and the e... |
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| Keywords: | methoxychlor calbindin-D9k mice uterus |
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