| 胃癌组织中Dnmt1基因mRNA和蛋白的表达 |
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| 引用本文： | 刘斌,顾立萍,邢传平,苏勤军,高自芳,钱震,董亮.胃癌组织中Dnmt1基因mRNA和蛋白的表达[J].第四军医大学学报,2009(20). |
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| 作者姓名： | 刘斌 顾立萍 邢传平 苏勤军 高自芳 钱震 董亮 |
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| 作者单位： | 兰州军区兰州总医院病理科;兰州大学基础医学院病理研究所; |
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| 基金项目： | 全军医药卫生科研基金(06MB102); 甘肃省自然科学基金(3ZS061-A25-094) |
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| 摘 要： | 目的:探讨正常胃黏膜、不典型增生胃黏膜及胃癌组织中Dnmt1基因mRNA及蛋白的表达以及两者之间的关系.方法:应用原位杂交技术及免疫组化SP法检测20例正常胃黏膜、40例不典型增生胃黏膜及60例胃癌组织中Dnmt1基因mRNA及蛋白的表达状况.结果:Dnmt1 mRNA在正常胃黏膜腺上皮中基本呈阴性表达(除部分增生的小凹上皮),其阳性率在正常胃黏膜、不典型增生胃黏膜及胃癌三组间呈递增趋势,组间有统计学差异(P<0.01).Dnmt1蛋白在正常胃黏膜腺上皮中基本呈阴性表达(除部分增生的小凹上皮和淋巴细胞),其阳性率在正常胃黏膜、不典型增生胃黏膜及胃癌三组间呈递增趋势,组间有统计学差异(P<0.01).Dnmt1 mRNA和蛋白的阳性表达与年龄、性别、肿物的大小、浸润深度、有无脉管转移等临床病理特征无关,而与有无淋巴结转移有关(P<0.05).Spearman等级相关分析显示Dnmt1蛋白与mRNA表达呈正相关(rs=1.000,P<0.01).结论:Dnmt1在胃癌组织中的高表达与胃癌的发生、发展及淋巴结转移密切相关.
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| 关 键 词： | 胃肿瘤 DNA甲基化转移酶1 免疫组织化学染色原位杂交 |
Expression of Dnmt1 gene mRNA and protein in gastric carcinoma |
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| LIU Bin,GU Li-Ping,XING Chuan-Ping,SU Qin-Jun,GAO Zi-Fang,QIAN Zhen,DONG Liang.Expression of Dnmt1 gene mRNA and protein in gastric carcinoma[J].Journal of the Fourth Military Medical University,2009(20). |
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| Authors: | LIU Bin GU Li-Ping XING Chuan-Ping SU Qin-Jun GAO Zi-Fang QIAN Zhen DONG Liang |
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| Institution: | LIU Bin1,2,GU Li-Ping2,XING Chuan-Ping1,SU Qin-Jun1,GAO Zi-Fang1,QIAN Zhen1,DONG Liang11Department of Pathology,Lanzhou General Hospital,Lanzhou Military Area Command,Lanzhou 730050,China,2Department of Pathology,Basic Medical College,Lanzhou University,Lanzhou 730000 |
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| Abstract: | AIM:To investigate the expression of Dnmt1 mRNA and its protein in normal gastric mucosa,atypical gastric hyperplasia,gastric carcinomas tissues and the relationship of both expression.METHODS:The expression of Dnmt1 mRNA and protein were respectively detected by SP immunohistochemistry and in situ hybridization in normal gastric mucosa(n=20),atypical gastric hyperplasia(n=40)and gastric carcinomas tissues(n=60).RESULTS:The positive expression of Dnmt1 mRNA was negative expression in normal gastric mucosa(e... |
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| Keywords: | gastric carcinoma Dnmt1 gene immunohistochemistry in situ hybridization |
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