| D-丝氨酸对大鼠视皮层神经元突触后NMDA受体功能的调制作用 |
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| 引用本文： | 李延海,王珏,张广军.D-丝氨酸对大鼠视皮层神经元突触后NMDA受体功能的调制作用[J].第四军医大学学报,2009(20):2098-2101. |
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| 作者姓名： | 李延海 王珏 张广军 |
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| 作者单位： | 西安交通大学生物医学信息工程教育部重点实验室生命科学与技术学院生物医学工程研究所; |
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| 基金项目： | 国家自然科学基金(30670660,10872156) |
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| 摘 要： | 目的：观察在发育大鼠视皮层脑片标本上D-丝氨酸对大鼠视皮层神经元突触后NMDA受体功能是否具有调制作用．方法：应用脑片膜片钳全细胞记录技术，记录13～15dSD大鼠视皮层Ⅱ-Ⅲ层椎体神经元的微小兴奋性突触后电流（mEPSCs），观察D-丝氨酸对mEPSCs的调制作用．结果：mEPSCs包含两种受体电流成分，即AMPA受体与NMDA受体电流成分；应用外源性D-丝氨酸（1，10和100μmol／L）均增强了mEPSCs的NMDA受体电流成分（P〈0．01），并呈现浓度依赖性，而应用NMDA受体阻断剂D—APV（50μmol／L）完全阻断这种增强效应．此外，D-丝氨酸没有影响mEPSCs的AMPA受体电流成分．结论：在大鼠视皮层Ⅱ-Ⅲ层锥体神经元上，突触后NMDA受体的甘氨酸结合位点是不饱和的，应用外源性D-丝氨酸可以增强NMDA受体功能，本研究为精神分裂等精神疾病的治疗提供有意义的资料．
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| 关 键 词： | NMDA受体 D-丝氨酸 微小兴奋性突触后电流 突触传递 视皮层 大鼠 |
D-serine modulates the postsynaptic NMDA receptor responses in the rat visual cortex |
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| LI Yan-Hai,WANG Jue,ZHANG Guang-JunKey Laboratory of Biomedical Information Engineering of Ministry of Education.D-serine modulates the postsynaptic NMDA receptor responses in the rat visual cortex[J].Journal of the Fourth Military Medical University,2009(20):2098-2101. |
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| Authors: | LI Yan-Hai WANG Jue ZHANG Guang-JunKey Laboratory of Biomedical Information Engineering of Ministry of Education |
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| Institution: | LI Yan-Hai,WANG Jue,ZHANG Guang-JunKey Laboratory of Biomedical Information Engineering of Ministry of Education,Institute of Biomedical Engineering,School of Life Science and Technology,Xi'an Jiaotong University,Xi'an 710049,China |
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| Abstract: | AIM:To investigate whether D-serine modulates the postsynaptic NMDA receptor responses in the rat visual cortex.METHODS:Whole cell patch-clamp recordings were used to record miniature excitatory postsynaptic currents(mEPSCs)from neurons in the visual cortex slices prepared from Sprague-Dawley rats aged 13-15 d.Then we examined whether exogenously applied D-serine modulates postsynaptic NMDA receptor responses.RESULTS:The mEPSCs were comprised of both NMDA and AMPA receptor-mediated components.Application of... |
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| Keywords: | NMDA receptors D-serine miniature excitatory postsynaptic currents synaptic transmission visual cortex rat |
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