葡萄糖、胰岛素对Colon26肿瘤细胞分泌VEGF的影响

目的：观察不同浓度的葡萄糖和胰岛素对结直肠癌细胞株Colon26肿瘤细胞分泌VEGF的影响．方法：体外培养Colon26肿瘤细胞，根据培养液中加入的葡萄糖和胰岛素终浓度的不同，将实验所用细胞分成12组．每组设3个复孔，培养48h，收集细胞培养上清，ELISA法检测VEGF的浓度．结果：B，C，D组（培养液分别加5，10，25mmol／L的葡萄糖）的VEGF浓度与A组（培养液未加葡萄糖和胰岛素，为对照组）的VEGF浓度相比无显著性差异（P〉0．05）．G组（培养液加125mU／L的胰岛素）的VEGF浓度明显高于A，E，F组（培养液分别加0，5，25mU／L的胰岛素）的VEGF浓度，差异有显著性（P〈0．01），而A组、E组与F组之间无显著性差异（P〉0．05）．K、L组（培养液分别加5，25mmol／L的葡萄糖和125，125mU／L胰岛素）的VEGF浓度均明显高于A组的VEGF浓度，差异有显著性（P〈0．01），而K、L组组间无差异．H，M，N组（培养液分别加5，5，1．25mU／L的胰岛素和5，25，25mmol／L的葡萄糖）的VEGF浓度与A组相比无显著性差异（P〉0．05）．结论：葡萄糖的浓度对Colon26肿瘤细胞分泌VEGF无明显影响．高浓度的胰岛素可增加Colon26肿瘤细胞VEGF分泌．

Effect of glucose and insulin on secretion of VEGF from Colon26 cells

AIM： To observe the effect of different concentrations of glucose and insulin on the secretion of VEGF in Colon26 tumor cells. METHODS： Colon26 tumor cells were cultured in vitro. According to the concentrations of glucose and insulin in culture medium, cells were divided into 12 groups. After added to different culture medium, cells were cultured for 48 h. In all experiments TGF-β1 concentration in the supernatant was detected by ELISA. RESULTS： In B, C, D groups （ including 5,10,25 mmol/L glucose in culture medium, respectively） , compared with A（culture medium, control）, there was no statistical difference about the secretion of VEGF （ P 〉 0.05 ）. In G group （ including 125 mU/L insulin in culture medium）, the level of VEGF was higher significantly than A, E and F groups （including 0, 5, 25 mU/L insulin in culture medium, P 〈 0. 01 ）. However, no differences between A, E and F groups were found. In K and L groups（ including 5, 25 mmol/L glucose and 125, 125 mU/L insulin in culture medium ）, the levels of VEGF were higher significantly than A （ P 〈 0.01 ）. However, no differences between K and L groups were found. In H, M and N groups（includlng 5, 5, 1.25 mU/L insulin and 5, 25, 25 mmol/L glucose in culture medium）, compared with A, there was no statistical difference about the secretion of VEGF（P 〉 0.05 ） . CONCLUSION ： The concentration of glucose have no effect on secretion of VEGF from Colon26 tumor cells. High concentration of insulin may increase secretion of VEGF from Colon26 tumor cells.