CT-1对癫痫持续状态大鼠海马神经元缺失及苔状纤维发芽的影响

目的：观察重组腺病毒心肌营养素1（Adv—CT1）对癫痫持续状态（SE）大鼠海马神经元缺失及苔状纤维发芽的影响，探讨CT-1对SE后脑损伤的修复作用．方法：建立氯化锂-匹罗卡品大鼠SE模型，随机将SE成功模型分为CT-1组（n=6，海马区注射Adv—CT1）及模型对照组（n=6，海马区注射生理盐水），另设正常对照组（n=6，腹腔注射生理盐水）．各组动物于SE后14d进行检测：①Nissl染色观察海马神经元形态学变化，并计数海马CA1区细胞数；②Timm染色检测苔状纤维发芽（MFS）程度；③免疫组化检测Caspase-3表达，结果用积分吸光度（IOD）值表示．结果：模型对照组可见海马区神经元变性、坏死，CT-1组病变较轻，正常对照组未见类似改变．模型对照组及CT-1组CA1区细胞数较正常对照组明显减少，但CT-1组细胞数明显多于模型对照组，差异具有统计学意义（P〈0．05）．模型对照组可见致密MFS染色颗粒；CT-1组虽也见MFS染色，但评分明显低于模型对照组（P〈0．05）．模型组Caspase-3的表达明显高于CT-1组（P〈0．05）．结论：CT-1可减轻SE后的神经细胞损伤，抑制MFS，其保护机制可能与抑制神经元凋亡，增加神经细胞存活有关．

Effect of cardiotrophin-1 on neuronal loss of hippocampus and mossy fiber sprouting in status epilepticus rats

AIM: To investigate the effect of recombinant adenovirus cardiotrophin-1(Adv-CT1) on the neuronal loss of hippocampus and the mossy fiber sprouting in status epilepticus(SE) rats and to determine whether CT-1 has protective effects on neuron damage after SE.METHODS: A lithium-pilocarpine induced SE model was used.SE-induced Wister rats were randomly divided into 2 groups(n=6,each): CT-1 group(injecting Adv-CT1 in hippocampus) and model control group(injecting saline in hippocampus),and another 6 rats served...