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无需离心微孔板凝聚胺法应用于新生儿溶血病检测效果评价
引用本文:车嘉琳,刘景春,陆小梅,邹文涛,刘赴平.无需离心微孔板凝聚胺法应用于新生儿溶血病检测效果评价[J].第四军医大学学报,2009(20):2168-2171.
作者姓名:车嘉琳  刘景春  陆小梅  邹文涛  刘赴平
作者单位:东莞市中心血站;东莞石龙博爱医院;
基金项目:东莞市医疗卫生科技计划基金(2007105150171)
摘    要:目的:评价无需离心的微孔板凝聚胺法在新生儿溶血病检测中的应用效果.方法:采用无需离心的微孔板凝聚胺法与传统的凝聚胺试管法及微柱凝胶卡技术3种方法同时进行新生儿溶血病检测.结果:3种方法进行单个新生儿溶血病检测,微柱凝胶卡时间最长,随着同时检测标本数量的增加,无需离心微孔板凝聚胺法检测速度有明显优势.3种方法对新生儿溶血病的检出能力没有显著性差异.结论:无需离心的微孔板凝聚胺法无需试管及离心设备,具有简便快速、经济有效等特点,是一种可行的新生儿溶血病检测方法.

关 键 词:凝聚胺  微孔板  无需离心  新生儿溶血病

Evaluation of microplate polybrene without a centrifuge in detecting hemolytic disease of newborn
CHE Jia-Lin,LIU Jing-Chun,LU Xiao-Mei,ZOU Wen-Tao,LIU Fu-Ping Dongguan Blood Center,Dongguan ,China,Dongguan Shilong Boai Hospital,Dongguan.Evaluation of microplate polybrene without a centrifuge in detecting hemolytic disease of newborn[J].Journal of the Fourth Military Medical University,2009(20):2168-2171.
Authors:CHE Jia-Lin  LIU Jing-Chun  LU Xiao-Mei  ZOU Wen-Tao  LIU Fu-Ping Dongguan Blood Center  Dongguan  China  Dongguan Shilong Boai Hospital  Dongguan
Institution:CHE Jia-Lin1,LIU Jing-Chun1,LU Xiao-Mei2,ZOU Wen-Tao1,LIU Fu-Ping1 1Dongguan Blood Center,Dongguan 523930,China,2Dongguan Shilong Boai Hospital,Dongguan 523321
Abstract:AIM:To evaluate the ability of microplate polybrene without a centrifuge(MPWC)in detecting hemolytic disease of newborn(HDN).METHODS:We used three methods,MPWC,Polybrene tube method(PTM)and Microcolumn gel technique(MGT),to detect hemolytic disease of newborn at the same time.RESULTS:Of the three methods,the time of testing,MGT is the longest,MPWC is longer than PTM when in detecting single,but with the increasing of samples,the MPWC is shorter than PTM.The ability of detecting HDN has no significant statis...
Keywords:polybrene  microplate  without centrifuge  hemolytic disease of newborn  
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