| 4个X-STR基因座复合扩增体系的建立及应用 |
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| 引用本文： | 白洁,刘华,李惠芬,陈庆,张庆霞,苏芹,唐晖,刘雅诚. 4个X-STR基因座复合扩增体系的建立及应用[J]. 医学争鸣, 2009, 0(20): 2053-2056 |
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| 作者姓名： | 白洁 刘华 李惠芬 陈庆 张庆霞 苏芹 唐晖 刘雅诚 |
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| 作者单位： | 中国科学院研究生院;北京市公安局刑侦总队法医检验鉴定中心;山西医科大学法医学院; |
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| 基金项目： | 北京市公安局科研项目(20050805) |
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| 摘 要： | 目的：建立DXS9902，DXS7130，DXS9898及DXS9895等4个X染色体特异性短串联重复（X—chromosome specific short tandem repeat，X-STR）基因座的复合扩增体系，调查其多态性并探讨在法医学实践中的应用．方法：复合扩增DXS9902，DXS7130，DXS9898及DXS98954个基因座，ABI PRISM 3100进行毛细管电泳检测，Genescan及Genetyper软件进行检测结果分析及等位基因分型．结果：在中国北方汉族200名无关男性个体及200名无关女性个体中，4个基因座分别发现了6，12，8，6个等位基因．结论：这4个基因座的复合扩增系统有较高的多态性信息，在个体识别和亲权鉴定中有较好的实用价值．
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| 关 键 词： | X染色体短串联重复 荧光复合扩增 遗传多态性 法医学应用 |
Development and forensic application of 4 X chromosome STR markers typing system |
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| BAI Jie,LIU Hua,LI Hui-Fen,CHEN Qing,ZHANG Qing-Xia,SU Qin,TANG Hui,LIU Ya-Cheng Post-graduate College,Chinese Academy of Sciences,Beijing ,China,Forensic Medical Examination , Identification Center,Beijing Public Security Bureau,Beijing ,School of Forensic Medicine,Shanxi Medical University,Taiyuan. Development and forensic application of 4 X chromosome STR markers typing system[J]. Negative, 2009, 0(20): 2053-2056 |
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| Authors: | BAI Jie LIU Hua LI Hui-Fen CHEN Qing ZHANG Qing-Xia SU Qin TANG Hui LIU Ya-Cheng Post-graduate College Chinese Academy of Sciences Beijing China Forensic Medical Examination Identification Center Beijing Public Security Bureau Beijing School of Forensic Medicine Shanxi Medical University Taiyuan |
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| Affiliation: | BAI Jie1,2,LIU Hua2,LI Hui-Fen3,CHEN Qing2,ZHANG Qing-Xia2,SU Qin2,TANG Hui2,LIU Ya-Cheng2 1Post-graduate College,Chinese Academy of Sciences,Beijing 100049,China,2Forensic Medical Examination and Identification Center,Beijing Public Security Bureau,Beijing 100192,3School of Forensic Medicine,Shanxi Medical University,Taiyuan 030002 |
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| Abstract: | AIM:To devise a multiplex PCR system of DXS9902,DXS7130,DXS9898 and DXS9895 and study the genetic polymorphism and application in forensic science.METHODS:DXS9902,DXS7130,DXS9898 and DXS9895 were amplified simultaneously using a multiplex system and were typed with ABI prism 3100 Genetic Analyzer by Genescan and Genetyper Analysis Softwares.RESULTS:A total of 200 male and 200 female individuals from North China Han population were tested;4 locus showed respectively 6,12,8,6 alleles.CONCLUSION:The 4 X-STR mu... |
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| Keywords: | X-STR fluorescent multiplex PCR genetic polymorphism forensic application |
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