| siRNA沉默CXCR4基因对食管鳞癌细胞EC9706体外侵袭能力的影响 |
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| 引用本文： | 王涛,轩小燕,臧文巧,李倩如,李敏,杨璇.siRNA沉默CXCR4基因对食管鳞癌细胞EC9706体外侵袭能力的影响[J].第四军医大学学报,2009(18). |
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| 作者姓名： | 王涛 轩小燕 臧文巧 李倩如 李敏 杨璇 |
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| 作者单位： | 河南中医学院第一附属医院血液肿瘤科;郑州大学基础医学院微生物学与免疫学教研室; |
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| 摘 要： | 目的:探讨siRNA沉默CXCR4基因对食管鳞癌EC9706细胞系体外侵袭能力的影响.方法:化学合成两对针对CXCR4基因的干扰序列siRNA1和siRNA2和一对荧光标记的阴性对照siRNA,转染人食管鳞癌EC9706细胞.荧光显微镜下观察转染效率,于转染后48 h采用半定量RT-PCR和Western Blot法检测各组细胞CXCR4 mRNA和蛋白表达水平的变化,Boyden侵袭小室检测各组细胞体外侵袭能力的变化.结果:与未转染组和转染阴性对照siRNA组相比,EC9706细胞转染两对CXCR4 siRNA48 h后,CXCR4 mRNA和蛋白表达水平明显下降(P<0.05),转染siRNA1组CXCR4 mRNA和蛋白下降尤为明显,未转染组和转染阴性对照siRNA组之间无明显差异(P>0.05).Boyden侵袭小室结果显示,转染CXCR4siRNA1和CXCR4 siRNA2组细胞穿膜数与未转染组和转染阴性对照siRNA组相比明显下降(P<0.05),未转染组和单纯转染脂质体转染试剂组之间无明显差异(P>0.05).结论:siRNA沉默CXCR4基因降低EC9706细胞CXCR4的表达和侵袭能力,为...
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| 关 键 词： | CXCR4 siRNA Boyden侵袭小室 食管鳞癌 |
Effect of siRNA target gene CXCR4 on invasion capability of EC9706 cells |
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| WANG Tao,XUAN Xiao-Yan,ZANG Wen-Qiao,LI Qian-Ru,LI Min,YANG Xuan.Effect of siRNA target gene CXCR4 on invasion capability of EC9706 cells[J].Journal of the Fourth Military Medical University,2009(18). |
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| Authors: | WANG Tao XUAN Xiao-Yan ZANG Wen-Qiao LI Qian-Ru LI Min YANG Xuan |
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| Institution: | WANG Tao1,XUAN Xiao-Yan2,ZANG Wen-Qiao2,LI Qian-Ru2,LI Min2,YANG Xuan21Department of Hemato-tumor,First Affiliated Hospital,Henan College of Traditional Chinese Medicine,Zhengzhou 450000,China,2Department of Microbiology and Immunology,Medical College of Basic Science,Zhengzhou University,Zhengzhou 450052 |
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| Abstract: | AIM: To explore the effect of silencing CXCR4 by siRNA on the invasion capability of esophageal carcinoma cell line EC9706 in vitro.METHODS: Two siRNAs targeting CXCR4 and one fluorescence-labeled siRNA as a negative control were chemically synthesized and were transfected into EC9706 cells.The transfection efficiency was observed under fluorescence microscope,CXCR4 mRNA and protein levels were detected by semi-quantitative RT-PCR and Western blot after 48 h,and the invasion capability of EC9706 cells in vi... |
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| Keywords: | CXC chemokine receptor 4 siRNA boyden chamber esophageal squamous carcinoma |
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