嗜酸粒细胞促淋巴细胞活性及抗肿瘤的作用机制

目的：探讨嗜酸粒细胞（EOS）体外抗肿瘤及对淋巴细胞活性的影响,研究EOS在肿瘤免疫中的作用.方法：建立豚鼠I型超敏反应模型,应用Percoll液不连续密度梯度法分离豚鼠血液、腹腔液中EOS.放射免疫法检测豚鼠血清及EOS与淋巴细胞共同培养的上清液（SELC）中TNF-α,IL-4含量;MTT法分别测定EOS,SELC,EOS裂解液（LLE）对白血病K562细胞,乳腺癌MCF-7细胞增殖活性的影响.结果：豚鼠血液、腹腔液EOS（1×10^6,1×107,1×10^8/L）能够促进脾淋巴细胞增殖,刺激指数最高分别可达1.92,2.58.血清,SELC中IL-4,TNF-α含量明显增高（P〈0.05）;腹腔液SELC48hIL-4,TNF-α含量最高分别为（3.94±0.16）,（8.93±0.15）g/L;腹腔液EOS,SELC,LLE与K562,MCF-7细胞共同培养,能够抑制K562,MCF-7细胞的增殖,尤其SELC对K562增殖的抑制率最高可达59.28%.结论：EOS可以促进淋巴细胞的增殖活性,提高SELC中IL-4,TNF-α的含量.腹腔液EOS,SELC,LLE能够抑制肿瘤细胞的增殖.

Antitumor and immunoregulation effects of eosinophils in vitro

AIM： To explore the antitumor effect of eosinophils （EOS） on K562 and MCF-7 ceils and the immumoregulation activity in lymphocytes. METHODS： Type I hypersensitivity model was established in Guinea pigs and eosinophils was isolaied from the peripheral blood and peritoneal fluid. Cytokines（ TNF-α, IL-4） in blood serum and in supernatant by coculture of eosinophil and lymphoeytes （SELC） were measured with radioimmunoassay, and the proliferation of K562 and MCF-7 cells treated with EOS, SELC and lysis liquid of eosinophil（LLE） was respectively assessed by tetrazolium salt reduetion（MTT） assay. RESULTS： The lymphocyte proliferation from the spleens in guinea pig was promoted with peripheral blood EOS and peritoneal fluid EOS（ 1 × 10^6, 1 × 10^7 , 1 × 10^8/L） , and the proliferation index was up to 1.92 and 2.58 respectively. The levels of TNF-α and IL-4 in SELC significantly increased （ P 〈 0.05 ） and up to （ 3.94 ± 0.16） and （8.93 ±0.15） g/L respectively. The proliferation of K562 and MCF-7 cells was respectively inhibited by peritoneal fluid EOS, SELC and LLE and the inhibition rate in the growth of K562 cells with SELC was the highest（ 59.28% ）. CONCLUSION： EOS promotes the lymphocytes proliferation activity and enhances the levels of TNF-α and IL-4 in SELC. The peritoneal fluid EOS, SELC, LLE inhibits the activity in tumor cells in vitro.