| 马兜铃酸致肾小管上皮细胞损伤中p53和Caspase-3活性的变化 |
| |
| 引用本文： | 许勇芝,陈丽萍,刘华锋,陶静莉,唐德燊.马兜铃酸致肾小管上皮细胞损伤中p53和Caspase-3活性的变化[J].第四军医大学学报,2009(15):1398-1401. |
| |
| 作者姓名： | 许勇芝 陈丽萍 刘华锋 陶静莉 唐德燊 |
| |
| 作者单位： | 广东医学院附属医院肾病研究所;深圳市福田区第二人民医院; |
| |
| 基金项目： | 广东省中医药局资助项目(104081) |
| |
| 摘 要： | 目的：研究马兜铃酸（AA）对人近端肾小管上皮细胞（HK-2）毒性作用中053和Caspase-3活性的改变，对其在AA肾毒性作用中的意义进行探讨．方法：用乳酸脱氢酶（LDH）释放试验测AA对HK-2的直接毒性作用，相差显微镜观察细胞形态学改变，Hoechsd3258染色法观察细胞凋亡形态的改变，流式细胞技术测细胞凋亡百分率，RT-PCR法测p53mRNA的表达水平，分光光度法测细胞Caspase-3活性改变．结果：AA浓度为80，160mg／L时，LDH释放率显著增高（P〈0．01）；10mg／LAA可诱导HK-2细胞凋亡百分率显著增高，AA为40mg／L时，细胞凋亡形态改变最明显和细胞凋亡比例最高（P〈0．01）；各组HK-2细胞053mRNA表达水平有统计学差异；40mg／LAA诱导HK-2细胞凋亡时，细胞内Caspase-3活性显著升高（与正常组比较，P〈0．01）．结论：AA能诱导HK-2细胞凋亡，其凋亡途径可能是非053依赖途径；凋亡过程中存在Caspase-3酶的激活．
|
| 关 键 词： | 马兜铃酸 肾小管上皮细胞 p53 Caspase-3 |
Changes of p53 and Caspase-3 in aristolochic acid-induced tubular epithelial cell apoptosis |
| |
| XU Yong-Zhi,CHEN Li-Ping,LIU Hua-Feng,TAO Jing-Li,TANG De-ShenInstitute of Nephrology,Affiliated Hospital,Guangdong Medical College,Zhanjiang ,China,Second People's Hospital,Futian District,Shenzhen.Changes of p53 and Caspase-3 in aristolochic acid-induced tubular epithelial cell apoptosis[J].Journal of the Fourth Military Medical University,2009(15):1398-1401. |
| |
| Authors: | XU Yong-Zhi CHEN Li-Ping LIU Hua-Feng TAO Jing-Li TANG De-ShenInstitute of Nephrology Affiliated Hospital Guangdong Medical College Zhanjiang China Second People's Hospital Futian District Shenzhen |
| |
| Institution: | XU Yong-Zhi1,CHEN Li-Ping2,LIU Hua-Feng1,TAO Jing-Li1,TANG De-Shen11Institute of Nephrology,Affiliated Hospital,Guangdong Medical College,Zhanjiang 524001,China,2Second People's Hospital,Futian District,Shenzhen 518049 |
| |
| Abstract: | AIM:To investigate the features of p53 expression and Caspase-3 activity assay in aristolochic acid(AA) induced renal tubular epithelial cell apoptosis.METHODS:The direct toxic effect of AA on HK-2 cells was evaluated by Lactate dehydrogenase(LDH) releasing essay.Apoptosis was observed under fluorescence microscope after the cells were stained by Hoechst 33258.The apoptosis rate of HK-2 cells induced by AA was estimated with flow cytometric analysis and the expression of wild-type p53 mRNA was measured by R... |
| |
| Keywords: | aristolochic acid renal tubular epithelial cells p53 Caspase-3 |
| 本文献已被 CNKI 维普 等数据库收录！ |
