| 慢病毒介导RNA干扰Cdh1的表达对大鼠脊髓损伤的修复作用 |
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| 引用本文： | 祁月红,钱巍,李平,姚文龙,万里,张传汉. 慢病毒介导RNA干扰Cdh1的表达对大鼠脊髓损伤的修复作用[J]. 医学争鸣, 2009, 30(15): 1353-1356 |
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| 作者姓名： | 祁月红 钱巍 李平 姚文龙 万里 张传汉 |
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| 作者单位： | 华中科技大学同济医学院附属同济医院麻醉学教研室,湖北,武汉,430030;华中科技大学同济医学院附属同济医院麻醉学教研室,湖北,武汉,430030;华中科技大学同济医学院附属同济医院麻醉学教研室,湖北,武汉,430030;华中科技大学同济医学院附属同济医院麻醉学教研室,湖北,武汉,430030;华中科技大学同济医学院附属同济医院麻醉学教研室,湖北,武汉,430030;华中科技大学同济医学院附属同济医院麻醉学教研室,湖北,武汉,430030 |
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| 基金项目： | 国家自然科学基金(30571788) |
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| 摘 要： | 目的：观察大鼠脊髓损伤后皮层体感运动区Cdh1 mRNA的表达变化，探讨慢病毒介导RNA干扰Cdh1的表达对脊髓损伤修复的作用．方法：15只雄性SD大鼠随机分成对照组和手术组，手术组采用Allen氏法建立脊髓打击损伤模型（T10-T11）．荧光定量PCR检测脊髓损伤后大鼠体感运动皮层区Cdh1 mRNA表达变化．30只雄性SD大鼠随机分为A组、B组和C组，建模1wk时分别注射生理盐水、空慢病毒和重组慢病毒．术后每周进行BBB评分，荧光定量PCR检测注射10d后Cdh1 mRNA的表达，损伤后6wk同法注射BDA．TR，8wk取脊髓冰冻切片．结果：手术组的Cdh1 mRNA表达高于对照组（P〈0．05），注射药物10d后C组Cdh1 mRNA表达低于A组及B组（P〈0．05）．损伤6wk后C组运动功能评分均高于另两组（P〈0．05），且在脊髓空洞区可见更多神经纤维通过．结论：APC—Cdh1在抑制轴突生长方面可能起着重要的作用．
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| 关 键 词： | 脊髓损伤 细胞周期末期促进复合物 慢病毒 RNA干扰 |
Lentivirus-mediated RNA interference inhibits Cdh1 expression in recovery of spinal cord injury in rats |
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| QI Yue-Hong,QIAN Wei,LI Ping,YAO Wen-Long,WAN Li,ZHANG Chuan-Han. Lentivirus-mediated RNA interference inhibits Cdh1 expression in recovery of spinal cord injury in rats[J]. Negative, 2009, 30(15): 1353-1356 |
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| Authors: | QI Yue-Hong QIAN Wei LI Ping YAO Wen-Long WAN Li ZHANG Chuan-Han |
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| Affiliation: | QI Yue-Hong,QIAN Wei,LI Ping,YAO Wen-Long,WAN Li,ZHANG Chuan-Han Department of Anesthesiology,Tongji Hospital,Tongji Medical College,Huazhong University of Science & Technology,Wuhan 430030,China |
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| Abstract: | AIM:To investigate the expression of Cdh1 in the sensorimotor cortical of rats after spinal cord injury and to study the repairing effect of silencing the expression of Cdh1 by lentivirus-mediated RNAi.METHODS:Fifteen male Sprague-Dawley rats were randomly divided into normal group and operation group.The injury models in the operation group were made with Allen method(T10-T11) and the expression of Cdh1 in the sensorimotor cortical was examined by quantitive real-time PCR.Another 30 male Sprague-Dawley rat... |
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| Keywords: | spinal cord injuries anaphase-promoting complex lentivirus RNA interference |
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