| 放射性碘-125辐射对液态AFP基因结构的影响 |
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| 引用本文： | 程木华,黄耀熊,吴正洁,黄宝天,刘志. 放射性碘-125辐射对液态AFP基因结构的影响[J]. 医学争鸣, 2009, 0(16) |
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| 作者姓名： | 程木华 黄耀熊 吴正洁 黄宝天 刘志 |
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| 作者单位： | 中山大学附属第三医院核医学科;暨南大学生物医学工程系; |
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| 基金项目： | 广东省自然科学基金(07001564); 广东省科技计划项目(2005B31201006) |
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| 摘 要： | 目的:探讨放射性碘-125(125I)辐射强度与DNA损伤的关系.方法:微量法激光拉曼光谱检测被不同浓度125I发射的俄歇电子辐射损伤液态甲胎蛋白基因(AFPDNA),分析DNA构象及碱基的拉曼光谱变化特点及特征峰相对强度变化规律.结果:125I发射的俄歇电子辐照AFP基因后,可见核糖、磷酸键及主链C-C构象的拉曼振动光谱峰偏移及峰值降低,并见嘌呤及嘧啶环的功能基团光谱峰降低.随125I浓度增加,逐渐出现DNA片段的特征拉曼光谱峰,并见核糖体暴露及碱基脱落堆积的新拉曼光谱峰.结论:125I辐照AFPDNA可引起DNA构象及功能基团损伤,随辐射强度增加逐渐出现碱基脱落及DNA结构破坏.
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| 关 键 词： | 激光 光谱分析 拉曼 俄歇电子 辐射 DNA |
Influence of radiation of radioactive iodine-125 on DNA structure of liquid AFP |
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| CHENG Mu-Hua,HUANG Yao-Xiong,WU Zheng-Jie,HUANG Bao-Tian,LIU Zhi. Influence of radiation of radioactive iodine-125 on DNA structure of liquid AFP[J]. Negative, 2009, 0(16) |
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| Authors: | CHENG Mu-Hua HUANG Yao-Xiong WU Zheng-Jie HUANG Bao-Tian LIU Zhi |
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| Affiliation: | CHENG Mu-Hua1,2,HUANG Yao-Xiong2,WU Zheng-Jie2,HUANG Bao-Tian2,LIU Zhi1 1Department of Nuclear Medicine,Third Hospital,Sun Yat-sen University,Guangzhou 510630,China,2Department of Biomedical Engineering,Jinan University |
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| Abstract: | AIM:To study the relation of the radiated intensity and the DNA damage induced by Auger electron.METHODS:The Alpha-fetoprotein DNA solution radiated by different doses of Auger electron emitting from the radioactive iodine-125 was examined using the Laser Raman spectroscopy.RESULTS:The differentiation of characteristic Raman vibration band of DNA was extensive and its peak intensity reduced after the DNA was radiated by Auger electron in 24 h.The Raman peak of the functional groups of pyridine and purine ba... |
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| Keywords: | lasers spectrum analysis raman auger electron radiation DNA |
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