| 同一组织不同层面的红外对比分析 |
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| 引用本文： | 王健生,张佳,吴文安,段小艺,王嗣岑,徐怡庄,石景森,吴瑾光,张海燕. 同一组织不同层面的红外对比分析[J]. 医学争鸣, 2009, 30(16): 1441-1444 |
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| 作者姓名： | 王健生 张佳 吴文安 段小艺 王嗣岑 徐怡庄 石景森 吴瑾光 张海燕 |
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| 作者单位： | 西安交通大学第一附属医院肿瘤外科;西安交通大学医学院,陕西,西安,710061;北京大学化学与分子工程学院,北京,100871;第二军医大学长海医院预防保健科,上海,200433 |
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| 基金项目： | 国家自然科学基金青年基金(30500119) |
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| 摘 要： | 目的：探讨同一生物组织在组织、细胞和细胞膜不同层面的傅立叶变换红外光谱（FTIR）谱图变化规律．方法：将提取肿瘤组织和瘤旁正常组织的细胞膜及提取细胞膜后剩余成份（沉淀物），于100kg／cm^2下8min制成溴化钾压片行FFIR检测，指认其特征光谱并将细胞膜、沉淀物、细胞株及组织的FFIR谱图进行对比分析．结果：细胞膜与沉淀物的FFIR谱图相比，蛋白和脂类的吸收峰发生显著变化，核酸的吸收峰在细胞膜消失而在沉淀物中增强；细胞膜的FTIR谱图与组织、细胞株相比，细胞膜的蛋白质二级结构发生了明显改变，脂类吸收峰增强；沉淀物的FrIR谱图与组织、细胞株相比，蛋白二级结构发生了明显改变，脂类吸收峰增强，但与组织谱图一致；癌组织中细胞膜、沉淀物及组织水平的相对强度I1460／I1400均小于正常组织．结论：同一组织不同层面的FTIR谱图变化不全一致；肿瘤组织中脂类含量减少，但细胞膜上的脂类含量增加，肿瘤组织及肿瘤组织细胞膜的蛋白质二级结构发生了明显改变；1460cm^-1和1400cm^-1可作为FrIR用于肿瘤诊断的一个可靠指标．
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| 关 键 词： | 谱学 傅里叶变换红外 胃肿瘤 脂类 蛋白质类 |
Comparative study on Fourier transform infrared spectroscopy of tissues at different tissue levels |
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| WANG Jian-Sheng,ZHANG Jia,WU Wen-An,DUAN Xiao-Yi,WANG Si-Cen,XU Yi-Zhuang,SHI Jing-Sen,WU Jin-Guang,ZHANG Hai-Yan. Comparative study on Fourier transform infrared spectroscopy of tissues at different tissue levels[J]. Negative, 2009, 30(16): 1441-1444 |
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| Authors: | WANG Jian-Sheng ZHANG Jia WU Wen-An DUAN Xiao-Yi WANG Si-Cen XU Yi-Zhuang SHI Jing-Sen WU Jin-Guang ZHANG Hai-Yan |
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| Affiliation: | WANG Jian-Sheng1,ZHANG Jia1,WU Wen-An1,DUAN Xiao-Yi1,WANG Si-Cen2,XU Yi-Zhuang3,SHI Jing-Sen1,WU Jin-Guang3,ZHANG Hai-Yan4 1Department of Oncology Surgery,First Affiliated Hospital,2School of Medicine,Xi'an Jiaotong University,Xi'an 710061,China,3College of Chemistry and Molecular Engineering,Peking University,Beijing 100871,4Department of Prevention and Health Care,Changhai Hospital,Second Military Medical University,Shanghai 200433 |
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| Abstract: | AIM:To investigate the Fourier transform infrared spectroscopy(FTIR)spectrum of the same tissue at the level of the tissue,the cell and the plasma membrane.METHODS:Plasma membrane and remaining ingredients were extracted from cancer tissues and normal tissues,and then compressed into thin KBr disks under a pressure of 100 kg/cm2 for 8 min for FTIR investigation.The spectra obtained from the cell line,tissue,plasma membrane and remaining ingredients were compared.RESULTS:Compared with that in tissue FTIR spe... |
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| Keywords: | spectroscopy fourier transform infrared stomach neoplasms lipids proteins |
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