| 凋亡抑制基因survivin真核表达载体的构建及其在SHG44细胞中的表达 |
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| 引用本文： | 张家墅,甄海宁,章翔,张瑞,韩涛,左毅,王鹏,霍军丽.凋亡抑制基因survivin真核表达载体的构建及其在SHG44细胞中的表达[J].第四军医大学学报,2009(16):1457-1460. |
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| 作者姓名： | 张家墅 甄海宁 章翔 张瑞 韩涛 左毅 王鹏 霍军丽 |
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| 作者单位： | 第四军医大学西京医院全军神经外科研究所;第四军医大学基础部免疫学教研室; |
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| 基金项目： | 国家自然科学基金(30672371) |
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| 摘 要： |
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| 关 键 词： | survivin 真核表达载体 神经胶质瘤 SHG44细胞 |
Construction of survivin eukaryotic expression vector and its expression in SHG44 cells |
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| ZHANG Jia-Shu,ZHEN Hai-Ning,ZHANG Xiang,ZHANG Rui,HAN Tao,ZUO Yi,WANG Peng,HUO Jun-Li PLA Institute of Neurosurgery,Xijing Hospital,Fourth Military Medical University,Xi'an ,China.Construction of survivin eukaryotic expression vector and its expression in SHG44 cells[J].Journal of the Fourth Military Medical University,2009(16):1457-1460. |
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| Authors: | ZHANG Jia-Shu ZHEN Hai-Ning ZHANG Xiang ZHANG Rui HAN Tao ZUO Yi WANG Peng HUO Jun-Li PLA Institute of Neurosurgery Xijing Hospital Fourth Military Medical University Xi'an China |
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| Institution: | ZHANG Jia-Shu1,ZHEN Hai-Ning1,ZHANG Xiang1,ZHANG Rui2,HAN Tao2,ZUO Yi1,WANG Peng1,HUO Jun-Li1 1PLA Institute of Neurosurgery,Xijing Hospital,Fourth Military Medical University,Xi'an 710033,China,2Department of Immunology,School of Basic Medicine |
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| Abstract: | AIM:To clone coding sequence of human survivin(SVV)gene,to construct its eukaryotic expression vector and to observe its expression in human brain astrocytoma cell line SHG44.METHODS:By RT-PCR,the specific coding sequence fragments of the SVV cDNA were amplified and cloned into eukaryotic expression vector pcDNA3.1.The eukaryotic expression vector pcDNA3.1-SVV was constructed and the gene transfection mediated by the lipofectin was used to introduce pcDNA3.1-SVV into human brain astrocytoma cell line SHG44.... |
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| Keywords: | survivin eukaryotic expression vectors glioma SHG44 cells |
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