| 不同转移潜能喉癌细胞株的分离及生物学特性比较 |
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| 引用本文： | 傅然,胡国华,汤为学.不同转移潜能喉癌细胞株的分离及生物学特性比较[J].第四军医大学学报,2009(19):1919-1922. |
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| 作者姓名： | 傅然 胡国华 汤为学 |
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| 作者单位： | 重庆医科大学附属第一医院耳鼻咽喉科;重庆市神经病学重点实验室; |
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| 基金项目： | 重庆市卫生局医学科学技术研究重点基金(08-1-16); 重庆市卫生局科研基金(07-2-07) |
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| 摘 要： | 目的：获得高侵袭性喉癌细胞株,为建立高转移喉癌动物模型奠定基础.方法：改良侵袭实验筛选出高低两种侵袭潜能细胞亚群,通过生长曲线、倍增时间和流式细胞术检测两种细胞的生长和周期差异;采用侵袭实验、迁移实验检测侵袭和迁移能力的差异;各取5只裸鼠进行动物实验检测体内肿瘤生长和转移能力的差异.结果：经过改良侵袭实验筛选出了高低两种侵袭潜能细胞亚群,倍增时间分别为（31.6±1.1）,（60.2±11.1）h（P〈0.05）;增殖指数分别为（27.6±0.5）%,（16.3±0.8）%（P〈0.01）;高低亚群侵袭细胞数分别为210.2±12.9和11.6±5.0（P〈0.01）,迁移细胞数分别为229.8±12.1和6.0±4.5（P〈0.01）.动物实验中,高低侵袭组在裸鼠体内的成瘤时间分别为2,4wk,病理活检高侵袭组3只同侧腘窝淋巴结转移、4只同侧腹股沟淋巴结转移、1只对侧腹股沟淋巴结转移;低侵袭组1只同侧腹股沟淋巴结反应性增生,2只同侧腹股沟淋巴结转移;瘤重分别为（17.0±3.0）,（9.7±3.8）mg（P〈0.05）;瘤内血管数分别为21.2±4.0,1.4±0.9（P〈0.01）.结论：成功从喉癌同一亲本细胞系中分离得到了高低两种侵袭潜能细胞亚群,高侵袭性亚群的恶性程度高于低侵袭性亚群.
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| 关 键 词： | 喉癌 侵袭 肿瘤转移 细胞系 |
Studies on metastatic biological characteristics of different metastatic potential human laryngeal carcinoma cell subclones |
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| FU Ran,HU Guo-Hua,TANG Wei-Xue.Studies on metastatic biological characteristics of different metastatic potential human laryngeal carcinoma cell subclones[J].Journal of the Fourth Military Medical University,2009(19):1919-1922. |
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| Authors: | FU Ran HU Guo-Hua TANG Wei-Xue |
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| Institution: | FU Ran1,HU Guo-Hua1,TANG Wei-Xue21Department of Otolaryngology,First Affiliated Hospital,Chongqing Medical University,Chongqing 400016,China,2Chongqing Key Laboratory of Neurology |
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| Abstract: | AIM:To isolate human laryngeal carcinoma cell subclones with different potential of metastasis.METHODS:High and low metastasis potential cell clones were isolated by improved invasion assay.Cell growth and cycle time were detected by cell growth curve assay,doubling time assay,and flow cytometry.Cell invasiveness and migration ability were analyzed by invasion assay and migration assay.Nude mice were used to study the growth and metastasis abilities in vivo.RESULTS:High and low metastasis potential cell clo... |
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| Keywords: | laryngeal carcinoma invasiveness neoplasm metastasis cell line |
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