| EPHB6受体型酪氨酸蛋白激酶对ERK信号通路的调控效应 |
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| 引用本文： | 铁茹,陈宝莹,刘利兵,陈健康,张学策,周幸春,曲萍,黄小军,于军.EPHB6受体型酪氨酸蛋白激酶对ERK信号通路的调控效应[J].第四军医大学学报,2009(19). |
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| 作者姓名： | 铁茹 陈宝莹 刘利兵 陈健康 张学策 周幸春 曲萍 黄小军 于军 |
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| 作者单位： | 第四军医大学基础部基础医学教学实验中心;第四军医大学唐都医院放射科; |
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| 基金项目： | 国家自然科学基金(30801385) |
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| 摘 要： | 目的:探讨促红细胞生成素产生肝细胞B6(EPHB6)对于胞外信号调节激酶(ERK)信号转导通路的作用.方法:以非小细胞肺癌细胞系A549作为研究对象,采用Western Blot法检测ERK的磷酸化水平及通路检测(PathDe-tect)法检测ERK下游分子和Ets结构域蛋白1(Elk-1)转录因子的活性.结果:A549细胞中EPHB6的表达导致ERK的磷酸化.相反,siRNA干涉EPHB6的表达可以逆转ERK的磷酸化.并且,EPHB6导致的ERK磷酸化与Elk-1转录因子脱偶联.结论:表明EPHB6对于ERK信号通路具有调控作用.
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| 关 键 词： | EPHB6 受体型酪氨酸蛋白激酶 非小细胞肺癌 细胞外信号调节激酶 有丝分裂原蛋白活化激酶 Elk-1 |
Regulation of EPHB6 receptor tyrosine kinase on the ERK signaling pathway |
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| TIE Ru,CHEN Bao-Ying,LIU Li-Bing,CHEN Jian-Kang,ZHANG Xue-Ce,ZHOU Xing-Chun,QU Ping,HUANG Xiao-Jun,YU JunCenter of Teaching Experiment,School of Basic Medical Science,Fourth Military Medical University,Xi'an ,China,Radiology.Regulation of EPHB6 receptor tyrosine kinase on the ERK signaling pathway[J].Journal of the Fourth Military Medical University,2009(19). |
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| Authors: | TIE Ru CHEN Bao-Ying LIU Li-Bing CHEN Jian-Kang ZHANG Xue-Ce ZHOU Xing-Chun QU Ping HUANG Xiao-Jun YU JunCenter of Teaching Experiment School of Basic Medical Science Fourth Military Medical University Xi'an China Radiology |
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| Institution: | TIE Ru1,CHEN Bao-Ying2,LIU Li-Bing1,CHEN Jian-Kang1,ZHANG Xue-Ce1,ZHOU Xing-Chun1,QU Ping1,HUANG Xiao-Jun1,YU Jun11Center of Teaching Experiment,School of Basic Medical Science,Fourth Military Medical University,Xi'an 710033,China,2Radiology Department of Tangdu Hospital,Xi'an 710038 |
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| Abstract: | |
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| Keywords: | NSCLC non-small cell lung cancer MAPK mitogen-activated protein kinase ERK extracellular-signal-regulated kinase DMSO dimethylsulfoxide FACS fluorescence-activated cell sorting |
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