季节变化对健康人免疫功能的影响

目的：动态观察季节变化对健康人免疫功能的影响.方法：对20例大学生志愿者分别在春分（VE）、夏至（SS）、秋分（AE）、冬至（WS）4个实验日分为VE组、SS组、AE组、WS组,应用流式细胞术对外周血CD3^＋,CD4^＋,CD8^＋,CD56^＋进行分析;应用放射免疫法检测血清中IL-1β,IL-2,IL-6及唾液中sIgA,IgG水平;应用酶联免疫吸附法检测血清中IFN-γ水平.结果：CD4＋VE组高于WS组（P〈0.05）;IL-2,IL-6高于AE组（5.39±1.17）vs（4.02±0.89）,（106.79±62.98）vs（95.87±39.43）,P〈0.05];IFN-γ（3.82±0.18）高于WS组（2.72±0.92）;CD3^＋和CD4^＋/CD8^＋比值SS组高于VE组、AE组、WS组（P〈0.05或P〈0.01）;CD8^＋而则低于后者,CD4^＋高于WS组（P〈0.01）;IL-1β,IL-2及IFN-γ高于AE组（0.21±0.15）vs（0.14±0.23）,（6.47±1.82）vs（4.02±0.89）,（4.58±0.21）vs（2.72±0.92）,P均〈0.05];IL-2,IFN-γ高于WS组（6.47±1.82）vs（3.55±1.02）,（4.58±0.21）vs（2.58±3.79）,P〈0.05或P〈0.01];sIgA,IgG高于WS组（95.98±56.35）vs（84.99±39.82）,（21.05±10.11）vs（18.03±9.27）,均P〈0.05].结论：机体免疫功能存在春夏高,秋冬低的季节节律,季节对免疫功能的调节可能是通过T淋巴细胞及其免疫网络进行的.

Effect of seasonal variation on basal immunity function of healthy individuals

AIM： To dynamically observe the influence of seasonal variation on the immune function in healthy individuals. METHODS： The immune substance was assessed in 20 healthy volunteers on Vernal Equino （ VE ） , Summer Solstice （ SS ） , Autumnal Equinox（AE） and Winter Solstice（WS） in their chronological order and the detected indexes were divided into 4 groups （VE, SS, AE and WS）. The populations of CD3^＋, CD4^＋, CD8^＋ and CD56^＋ lymphocytes from peripheral blood were assessed by flow cytometry. The levels of IL-1β, IL-2 and IL-6 from serum and sIgA and IgG from saliva were measured by RIA. The levels of IFN-γ from serum were detected by ELISA. RESULTS： Compared with those in VE group, the populations of CD4^＋ and the levels of IFN-γ were obviously lower in WS group （P 〈 0.05 ） and the levels of IL-2 and IL-6 were lower in AE group（ P 〈 0.05 ）. Compared with those in SS group, the populations of CD3^＋ and the CD4^＋/CD8^＋ ratios were lower in VE, AE and WS groups, while the populations of CD8^＋ increased significantly（ P 〈 0.05 or P 〈 0.01 ） in these groups. The populations of CD4^＋ reduced in WS group（P 〈0.01 ） , the levels of IL-1β, IL- 2 and IFN-γ decreased in AE group（P 〈0.05 ） and the levels of IL-2, IFN-γ sIgA and IgG reduced obviously in WS group （ P 〈 0.05 or P 〈 0.01 ）. CONCLUSION： There is a tendency that immune function is higher in spring/summer and lower in autumn/winter, and the effect of seasonal variation on immunity function is mainly regulated by T lymphocytes and their immunological network.