| 高压氧对缺氧缺血性脑损伤新生大鼠caspase-3及caspase-9表达的影响 |
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| 引用本文： | 牛彦平,王玲,檀国军,姜建勇.高压氧对缺氧缺血性脑损伤新生大鼠caspase-3及caspase-9表达的影响[J].第四军医大学学报,2009(20):2112-2115. |
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| 作者姓名： | 牛彦平 王玲 檀国军 姜建勇 |
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| 作者单位： | 河北医科大学第二医院康复科;河北医科大学第三医院神经内科;河北医科大学第二医院神经内科; |
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| 基金项目： | 河北省科委科技攻关计划项目(052761527) |
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| 摘 要： | 目的：通过检测caspase-3，caspase-9的表达情况，探讨高压氧（HBO）对缺氧缺血新生大鼠脑细胞凋亡的影响及分子机制．方法：建立缺氧缺血性脑损伤（HIBD）大鼠模型，将其分为HIBD组（24只）及HBO干预组（24只），同时设立假手术（Sham）组（24只）．分别于造模后18，24，48和96h4个时间点断头取脑观察其病理改变（海马及皮层区），并用免疫组化的方法检测caspase-3及caspase-9的蛋白表达．结果：HIBD组18，24，48和96h时间点海马及皮层区caspase-3，caspase-9蛋白的表达均明显高于Sham组（P〈0．05），HBO干预组则显著缓解，caspase-3，caspase-9表达的升高程度均低于HIBD组（P〈0．05）．结论：HBO能减轻新生大鼠HIBD模型海马及皮层区脑细胞的凋亡，其作用可能是通过下调caspase-9进而影响caspase-3的活性表达来完成的．
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| 关 键 词： | caspase-3 caspase-9 凋亡 高压氧 缺氧缺血性脑损伤 |
Effect of hyperbaric oxygen-treatment on the expression of caspase-3 and caspase-9 in the newborn rats brain of hypoxic-ischemia |
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| NIU Yan-Ping,WANG Ling,TAN Guo-Jun,JIANG Jian-Yong.Effect of hyperbaric oxygen-treatment on the expression of caspase-3 and caspase-9 in the newborn rats brain of hypoxic-ischemia[J].Journal of the Fourth Military Medical University,2009(20):2112-2115. |
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| Authors: | NIU Yan-Ping WANG Ling TAN Guo-Jun JIANG Jian-Yong |
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| Institution: | NIU Yan-Ping1,WANG Ling2,TAN Guo-Jun3,JIANG Jian-Yong11Department of Rehabilitation,Second Affiliated Hospital,2Department of Neurology,Third Affiliated Hospital,3Department of Neurology,Hebei Medical University,Shijiazhuang 050000,China |
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| Abstract: | AIM:To explore the effect of Hyperbaric Oxygen(HBO)on the apoptosis resulted from Hypoxic-Ischemic Brain Damage(HIBD),and to observe the change of Caspase-3 and Caspase-9 expression in this process.METHODS:The hypoxia-ischemia models were divided into following 3 groups:HIBD model group(24 cases),HBO treatment group(24 cases)and sham operation group(24 cases).The brain were taken from the model at 18,24,48 and 96 h after the model was made by decapitation.Then observe the morphologic change of brain tissues... |
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| Keywords: | caspase-3 caspase-9 apoptosis hyperbaric oxygen hypoxic-ischemic brain damage |
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