与TLRs通路相关的炎性反应因子在大肠癌中的表达及意义

目的 探讨与Toll样受体(Toll-like receptors,TLRs)通路相关的炎性反应因子在大肠癌中的临床意义.方法 收集56例大肠癌组织和36例结肠腺瘤组织,以癌旁正常肠黏膜组织为对照,应用免疫组织化学法检测TLR2、核因子κB(nuclear factor κB,NF-κB)及白细胞介素8(interleukin 8,IL-8)的表达情况,并分别找出与大肠癌临床病理特征的相关关系.结果 在大肠癌组织、癌旁正常组织及结肠腺瘤组织中TLR2蛋白的阳性表达率分别为71.4％、42.9％、58.3％,NF-κB的阳性率分别为78.6％、46.4％、83.3％,IL-8阳性率分别为75.0％、46.4％、72.2％,3种组织中的表达差异均具有统计学意义(均P＜0.05);大肠癌组织中NF-κB的表达与TLR2及IL-8密切相关(P＜0.05);此外,大肠癌组织中TLR2的表达与分化程度、有无淋巴结转移及TNM分期相关(P＜0.05),NF-κB及IL-8的表达与脉管受侵、有无淋巴结转移及TNM分期相关(P＜0.05).结论 TLRs通路介导的慢性炎性反应因子的高表达与大肠癌的临床病理特征密切相关,提示TLRs信号通路在大肠癌的发生、发展中有重要的作用.

Expression of inflammatory factors of Toll like receptor signaling pathway in colorectal carcinoma and its clinical significance

Objective To investigate the expression of inflammatory factors of Toll like receptor （TLR） signaling pathway in coloreetal carcinoma. Methods The expression of TLR2, nuclear factor KB （ NF-KB ） and interleukin-8 （ IL-8 ） was detected by immunohistochemistry in 56 samples of colorectal cancer,36 samples of colorectal adenoma tissue and 56 samples of pericancerous normal colorectal tissue. The correlation of TLR2, NF-κB and IL-8 expression with elinicopathological features of colorectal cancer was analyzed. Results In colorectal carcinoma tissue, adjacent normal colorectal tissue and colorectal adenoma tissue, the positive expression rates of TLR2 were 71.4% ,42.9% and 58.3 %, the positive rates of NF-KB were 78.6% ,46.4% and 83.3%, and the positive rates of IL-8 were 75.0% ,46.4% and 72. 2% ,respectively（ all P 〈 0.05 ）. The expression of NF-KB was closely correlated with the expression of TLR2 and IL-8 （P 〈 0. 05 ）. In addition, the expression of TLR2 was correlated with tumor differentiation, lymph node metastasis and clinical stages（ P 〈 0. 05 ）. The expression of NF-KB and IL-8 was correlated with vascular invasion,lymph node metastasis and clinical stages （ P 〈 0.05 ）. Conclusion Overexpression of inflammatorj factors of Toll like receptor signaling pathway is closely correlated with the biological behavior of colorectal cancer, suggesting that TLR signaling pathway may be involved in the pathogenesis and development of colorectal cancer.