N-乙酰半胱氨酸治疗猪急性肝衰竭的作用

目的研究N-乙酰半胱氨酸(NAC)对D-氨基半乳糖诱导的急性肝衰竭动物肝脏的保护作用。方法雌性中国实验小型猪14头。随机分为对照组(6头)和治疗组(8头),应用D-氨基半乳糖1.2g/kg,静脉滴注,诱导制备药物性急性肝衰竭动物模型。治疗组给予NAC治疗,对照组同期给予生理盐水。观察比较2组动物一般状况、生存时间、生理指标、生化指标以及肝脏组织病理变化。结果经过治疗后,治疗组在某些时间点与对照组相比部分肝功能指标明显改善:ALT、AST和总胆红素含量明显较低,凝血酶原活动度较高,凝血酶原时间缩短;其他生化指标,如血氨和内毒素水平有一定下降;在组织病理学上,治疗组与对照组相比炎症有所减轻。结论NAC治疗非对乙酰氨基酚引起的肝衰竭仍然可以改善肝功能和炎症反应,内毒素血症得到一定改善。

Protective effects of N-acetylcysteine on pigs with acute liver failure

Objective To observe the protective effects of N-acetylcysteine (NAC) on pigs with acute liver failure induced with D-galactosamine. Methods Fourteen minipigs were divided into two groups at random,control group(n=6)and treatment group(n=8). All of the animals were induced to acute liver failure with administration of D-galactosamine (1.2 g/kg of dosage). Then the treatment group was treated with NAC,and the control group was dealed with saline solution. The efficacy was assessed by survival time,improvements in manifestation,biochemical and pathological parameters in both groups. Results Compared with the control group,liver function indexes were obviously improved at some time points in the treatment group. ALT,AST and total bilirubin levels were reduced greatly,prothrombin activity (PTA) was increased and prothrombin time(PT) was shortened. There was a decrease in other biochemical indexes,such as blood ammonia and endotoxin. Inflammation was reduced histopathologically in the treatment group. Conclusion N-acetylcysteine can effectively improve liver functions and reduce inflammation in acute liver failure induced by paracetamol ,and improve endotoxemia as well.