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| 在大肠杆菌中融合高效表达人MCP-1 | |
| 引用本文： | 张毅,叶棋浓,刘及,苏国富,滕家波. 在大肠杆菌中融合高效表达人MCP-1[J]. 吉林大学学报(医学版), 1999, 25(6): 685-687 |
| 作者姓名： | 张毅 叶棋浓 刘及 苏国富 滕家波 |
| 作者单位： | 1. 白求恩医大预防医学院毒理教研室,长春,130021 2. 军事医学科学院吉林大学学院所 3. 吉林大学医学院卫生部长春生物制品研究所 |
| 摘 要： | 目的：利用含强启动子的融合表达载体ｐＧＥＸ－２Ｔ对编码人单核细胞趋化蛋白－１（ＭＣＰ－１）的基因进行高效表达。方法：基因重组技术及蛋白纯化技术。结果：ＳＤＳ－ＰＡＧＥ显示表达产物占菌体蛋白的５０％ 。Ｗｅｓｔｅｒｎ Ｂｌｏｔ检测表明，表达产物可与抗ＭＣＰ－１ 抗体特异反应。生物学活性测定结果表明，表达产物具有明显的单核细胞趋化活性。结论：融合蛋白对ＭＣＰ－１ 趋化活性无影响 |
| 关 键 词： | 人单核细胞趋化蛋白-1 高表达 趋化活性 |
| 修稿时间： | 1998-11-16 |
High level expression of MCP-1 in the form of fusion protein in E.coli |
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| Zhang Yi,Ye Qinong,Liu Ji,Su Guofu,Teng Jiabo. High level expression of MCP-1 in the form of fusion protein in E.coli[J]. Journal of Jilin University: Med Ed, 1999, 25(6): 685-687 | |
| Authors: | Zhang Yi Ye Qinong Liu Ji Su Guofu Teng Jiabo |
| Abstract: | Objective:A new overexpression plasmid pGEX 2T/MCP 1 was constructed,in which a gene fragement encoding human monocyte chemoattractant protein 1 (MCP 1) was inserted downstream of tac promoter.Methods:DNA recombinant technique was used.Results:The expression level was 50% of total bacterial proteins by SDS PAGE.Western blot analysis showed that the expressed products reacted specifically with anti MCP 1 antibodies.Detection of activity by the method of agarose plates showed that the expression product had obvious monocyte chemoattractant activity.Conclusion:Fusion protein may not affect the chemoattractant activity of MCP 1. |
| Keywords: | MCP-1 high expression chemoattractant |
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