肌苷对大鼠脑缺血再灌注后GAP-43 mRNA表达影响

目的探讨肌苷对脑缺血再灌注后中枢神经再生的作用。方法线栓法建立大脑中动脉缺血再灌注模型,随机分为治疗组和对照组,每组再随机分为缺血1.5 h再灌注2 h、12 h、1 d、2 d、3 d、7 d1、4 d组(n=4),另取4只作假手术组。应用BEDERSON等神经功能评分法评定神经功能,原位杂交技术检测脑缺血再灌注后各时间点脑组织生长相关蛋白基因(GAP-43 mRNA)的表达。结果对照组于再灌注2 h~7 d、治疗组于再灌注12 h~7 d,GAP-43 mRNA表达与假手术组比较明显增多(t=2.70~29.84,P<0.05),治疗组与对照组比较GAP-43mRNA表达于再灌注2 h一过性下降,12 h和7 d明显增高(t=1.97~7.41,P<0.05);治疗组与对照组比较神经功能恢复于再灌注7 d有显著性差异(t=2.31,P<0.05)。结论肌苷可促进大鼠脑缺血再灌注后神经功能恢复,其作用机制可能是通过调节与神经轴突再生有关的GAP-43 mRNA表达而实现的。

EFFECTS OF INOSINE ON EXPRESSION OF GAP-43 mRNA AFTER CEREBRAL ISCHEMIC REPERFUSION IN RATS

ObjectiveTo explore the effects of inosine on central nervous regeneration after cerebral ischemic reperfusion.MethodsThe model of the middle cerebral artery occlusion(MCAO) in SD rats was established by nylon monofilament suture.The rats were randomly divided into treated and control groups,and they were further divided into 2-, 12-hour,1-,2-,3-,7-and 14-day reperfusion subgroups.The other four rats were sham-operated.BEDERSON and other neurological grading were used to investigate the nerve functions and in situ hybridization for the expression of GAP-43 mRNA in brain tissue.Results(The GAP-43 mRNA) expressions at two hours to seven days of reperfusion in the control group and at 12 hours to seven days of reperfusion in the treated group were more significant compared with those in the sham-operated group(t=2.70-29.84,P<(0.05));but at two hours of reperfusion it was less expressed,and at 12 hours and seven days of reperfusion it was more significantly expressed in the treated group than that in the control group(t=1.97-7.41,P<0.05).Neurologic grade at seven days of reperfusion increased significantly compared with that in the control group(t=2.31,P<0.05).ConclusionInosine can improve neurologic grade of MCAO rats.The enhanced neurological function might partially result from the up-regulation of GAP-43 mRNA,which is correlated with neuronal regeneration.