栀子药材中三种有效成分HPLC定量分析结果不确定度的评定

采用HPLC方法同时测定栀子药材中栀子苷、绿原酸和藏红花素Ⅰ的含量,通过对测试方法流程进行分析,确定不确定度来源,结合含量测定方法验证数据,计算了不确定度分量、合成不确定度及扩展不确定度;结果表明,用HPLC方法定量分析栀子药材中栀子苷、绿原酸和藏红花素1的方法满足现代药物分析方法学的要求,且三者的扩展不确定度分别为0.1024,0.2254,0.1265.

Estimation of Measurement Uncertainty of Analytical Results for the Determination of Three Active Components from Gardenia jasminoides Ellis.by HPLC

Objective: To estimate the uncertainty for quantitative analysis results of geniposide,chloregenic acid and crocinl I in Gardenia jasminoides Ellis.Methods: HPLC method was employed to determine the amounts of geniposide,choloregenic acid and crocinl I in Gardenia jasminoides Ellis.Analyzing the uncertainty sources arising from the procedure of analysis,the standard uncertainty and combined uncertainty and expanded uncertainty were calculated according to the data of HPLC.Results: This method met the requirements of modern pharmaceutical analysis,and the expand uncertainties for the HPLC method of the three components are 0.1024,0.2254,0.1264,respectively.Conclusion: Applying measurement uncertainty to the evaluation of quantitative analysis results of active components in Gardenia jasminoides Ellis.is an improvement to the actual error evaluation system.