泰乐菌素产生菌的空间诱变育种研究

目的研究弗氏链霉菌(streptomyces fradioe)在空间条件下的变异规律,并筛选高产菌株应用于工业生产。方法将弗氏链霉菌(streptomyces fradiae)9940S^ _86连续经“神舟”1、3、4号飞船搭载进行太空诱变育种,统计筛选结果,初步探讨泰乐菌素产生菌在空间条件下的变异规律。结果复筛后得到48株效价较出发菌株提高20％以上的菌株,其中总效价最高达到14950μg／ml(摇瓶),较出发菌株提高91．5％。结论弗氏链霉菌的空间诱变受飞行时间影响较大。菌株经多次搭载后其产量变异有累积作用,并且产量变异和形态变异存在相关性。通过中试、生产实验,最终选定产量较出发生产菌株提高18％的T1—156—84—23菌株投入试生产。

A Study on Space Mutation of Streplomyces Fradiae

Objective: To study the rule of mutation of Streptomyces fradiae during spaceflight, and to select efficient tylosin producing strains for industrial production. Method: Streptomyces fradiae 9940S(+)-86 were carried on-board spaceship "Shenzhou" I, "Shenzhou" III and "Shenzhou" IV sequentially to achieve spaceflight mutation breeding experiment. Result: After space experiments and the screening tests in the lab, 48 strains were obtained which promoted production by +20% or more at shaker level. And the highest production of a strain was 14950 micrograms/ml, which means an increase of 91.5%. Conclusion: Comparing the results of three tests, it is found that the outer space environment can lead to a cumulative mutation. After the medium scale tests and production experiments, strain T1-156-84-23 was finally selected to be used for sample production. And its output was increased by 18%.