基于超高效液相-飞行时间质谱技术的 中药女贞子代谢组学研究

目的 :建立中药女贞子生品及不同工艺炮制品的代谢组学分析方法,比较研究其指纹图谱差异,探讨女贞子炮制机制。 方法 :采用超高效液相-飞行时间质谱(UPLC-Q-TOF-MS)及多元统计分析技术对生品及不同工艺炮制品进行指纹图谱差异比较分析。 结果 :生品和不同炮制品指纹图谱存在明显差异,且鉴定了一个产生差异的化学成分标志物- ligustaloside B,其在女贞子炮制品中的含量高于女贞子生品。 结论 :UPLC-Q-TOF-MS结合多元统计分析方法可对女贞子药材及其饮片进行质量评价,同时为炮制机制提供了一定依据。

Ultra-performance LC/TOF MS Analysis of Fruits of Ligustrum lucidum for Metabolomic Research

Objective: To establish an ultra-performance LC-quadrupole TOF MS (UPLC-Q-TOF-MS) method of the crude and processed fruits of Ligustrum lucidum for metabolomic research, comparative study in their metabolite profiling was performed to explore the mechanism of processing the fruits of L. lucidum. Method: Metabolite profiling of crude and processed fruits of L. lucidum were performed using UPLC-Q-TOF-MS and multivariate statistical analysis technique. Results: There is significant difference of metabolite profiling among the crude and different processed fruits of L. lucidum. The chemical markers such as ligustaloside B for such variations was identified. And its contents in the crude L. lucidum was significantly higher that in the processed one. Conclusions: This study indicated that UPLC-Q-TOF-MS coupled with multivariate statistics is able to provide quality control of the crude and processed fruits of L. lucidum. While the results provide the basis for the mechanism of processing.