小儿喘息性疾病患者血清sCD86及IgE水平

目的：了解血清可溶性CD86（soluble CD86,sCD86）、总IgE（total immunoglobulin E,tIgE）及特异性IgE（specific immunoglobulin E,sIgE）在喘息性疾病中的表达,为临床治疗喘息性疾病提供理论依据。方法：采用ELISA法检测54例喘息性疾病与20例健康对照血清sCD86及tIgE水平,采用欧蒙印迹法检测45例喘息性疾病与17例健康对照血清变应原sIgE。结果：喘息性疾病组sCD86比对照组明显升高（P（0.001）,毛细支气管炎、喘息性支气管炎、支气管哮喘三组之间无显著性差异（P〉0.05）;喘息性疾病组tIgE比对照组明显升高（P〈0.001）,tIgE升高率也明显升高（P（0.01）;喘息性疾病组变应原以sIgE的阳性率明显高于对照组（P〈0.05）;喘息性疾病吸入性变应原sIgE以屋尘、尘螨组合最多见,食物性变应原以淡水鱼最常见;tIgE和sIgE有较好的相关性（P〉0.05）;喘息性疾病血清sCD86的浓度与tIgE未显相关性（P〉0.05）,喘息性疾病变应原sIgE阳性组与sIgE阴性组sCD86的浓度无显著性差异（P〉0.05）。结论：sCD86在喘息性疾病发病中有一定的作用,小儿喘息性疾病与Ⅰ型变态反应关系密切。

A Preliminary Study on Serum Levels of sCD86 and IgE of Children with Wheezy Diseases

Objective：To explore the role of serum soluble CD86（sCD86）,total immunoglobulin E（tIgE） and specific immunoglobulin E（sIgE） in immune pathogenesis of wheezy diseases in children.Methods： Serum sCD86 and tIgE of 54 children with wheezy diseased（group D1） and 20 healthy children（group C1） were tested with ELISA method,and sIgE of 45 wheezy disease cases（group D2） and 17 healthy children（group C2） were determined with EUROLINE method.Results： Serum sCD86 and tIgE levels of group D1 were higher than those of group C1 significantly（P﹤0.001）,but there was no significant difference among three types of wheezy diseases（P0.05）.The increase rate of tIgE of group D1 was much higher than that of group C1（P﹤0.01）.The positive rate of sIgE in group D2 was much higher than that of group C2（P0.05）.The inhalant allergen serum sIgE of wheezy disease patients was mainly house dust and dust mite mix;Food allergen was usually fresh water fish.There was a positive correlation between tIgE and sIgE levels（P0.05）,while no correlation was found between the concentrations of serum sCD86 and tIgE in wheezy disease patients（P0.05）.There was no difference of sCD86 between sIgE-positive and sIgE-negative cases of wheezy disease（P0.05）.Conclusions： sCD86 plays an important role in development of wheezy diseases in children.There is a close relationship of wheezy diseases of children to type I allergy.