16SrRNA基因在快速诊断新生儿败血症的病原菌研究

目的探讨血液16SrRNA基因检测在新生儿败血症诊断中的应用价值。方法分析细菌16SrRNA基因保守区，设计一对通用引物扩增已知实验菌株，检测其特异性，用倍比稀释法检测其敏感性，同时进行血培养。结果已知实验菌株均获得920bp扩增产物，对照组中的人类基因组DNA、HBV—DNA和白色假丝酵母菌无相应产物。敏感性测试能达到lpg大肠杆菌DNA。PCR阳性率为31．7％（20／63），血培养阳性率为14．3％（9／63），两者比较有显著性差异（P〈0.05）。结论PCR检测血液细菌16SrRNA基因，具有特异性强，敏感度高等特点，能在临床推广应用。

Research on a rapid method for the detection of bacteia in neonatal septicemia by using 16S rRNA gene

Objective： To explore the significance of 16S rRNA gene in diagnosis of neonatal septicemia. Methods： We analysed bacterial 16S rRNA genes in conservative districts and designed a pair of universal primers for amplification of a known experimental strains for specific detection, with double dilution assay for detection of sensitivity, and blood culture was conducted simultaneously. Results ： The known experimental strains were amplified and products were 920bp, but human genome DNA, HBV - DNA and candi- da albicans showed no corresponding products. Sensitivity test showed that it could detect as low as lpg of E. coli DNA. The positive rate of PCR was 31.7% （20/63）, the positive rate of blood culture was 14. 3% （9/63）, more significant differences between the two group （P 〈 0. 05 ）. Conclusion ： The technology of PCR has strong specificity and high sensitivity, it can be used for clinical.