| 一种新型多拷贝毕赤酵母表达载体的构建 |
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| 引用本文： | 蒋燕明,杨治华,冉宇靓,李力. 一种新型多拷贝毕赤酵母表达载体的构建[J]. 广西医科大学学报, 2005, 22(4): 511-513 |
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| 作者姓名： | 蒋燕明 杨治华 冉宇靓 李力 |
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| 作者单位： | 广西医科大学附属肿瘤医院妇科,南宁,530021;中国医学科学院肿瘤研究所 |
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| 摘 要： | 目的：构建合适的载体用于外源基因在巴斯德毕赤酵母中的正确分泌表达。方法：利用基因重组技术，对已有载体pPIC9K进行改造：除去信号肽末端的XhoI酶切位点，在多克隆位点处添加此切点；添加一个多聚组氨酸纯化标签；重建了阅读框架。结果：成功构建了一个多拷贝毕赤酵母表达载体。结论：可以利用这个表达载体对外源基因进行分泌表达。
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| 关 键 词： | 巴斯德毕赤酵母 表达 载体 |
| 收稿时间： | 2005-02-21 |
| 修稿时间： | 2005-02-21 |
CONSTRUCTION OF A NEW MULTI-COPY PICHIA EXPRESSION VECTOR |
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| Jiang Yanming,Yang Zhihua,Ran Yuliang,. CONSTRUCTION OF A NEW MULTI-COPY PICHIA EXPRESSION VECTOR[J]. Journal of Guangxi Medical University, 2005, 22(4): 511-513 |
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| Authors: | Jiang Yanming Yang Zhihua Ran Yuliang |
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| Affiliation: | the Affiliated Cancer Hospital of Guangxi Medical University, Nanning 530021 China |
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| Abstract: | Objective:To construct suitable vectors for the secretory expression of heterologous proteins in Pichia pastoris.Methods:A restriction site XhoI was eliminated in the end of secretion signal,another restriction site XhoI was added in multiple cloning site;Polyhistidine 6xHis was added before multiple cloning sites and the reading frame was reconstructed.Result:A new multi-copy vector pPEX9k derived from pPIC9K was successfully constructed.Conclusion:The vector pPEX9k is suitable for the secretory expression of heterologous proteins. |
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| Keywords: | Pichia pastoris expression vector |
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