羧甲基壳聚糖/环丙沙星植入缓释微球防治局部感染的实验研究

目的:探索局部植入抗菌药缓释微球防治术后局部感染的可行性及效果.方法:以羧甲基壳聚糖为缓释辅料,通过乳化交联工艺制备负荷盐酸环丙沙星的植入缓释微球;建立模拟体内释放环境的体外释放系统,评价微球体外释放特性和抑菌能力;利用高效液相色谱法检测药物的体内释放特性;利用透射电镜和病理检查检测微球的体内降解和组织相容性.结果:羧甲基壳聚糖环丙沙星植入缓释微球在体外的释放达7 d以上;微球植入体内后局部药物浓度维持在金葡菌MIC90以上约10 d,无明显突释现象;微球植入动物体内后无局部感染和切口愈合不良;透射电镜和病理结果发现微球和组织之间无明显的炎性异物反应.结论:羧甲基壳聚糖环丙沙星植入缓释微球在体内具有良好的生物相容性和释药行为,可用于植入体内防治局部感染.

Prevention and treatment of local infection using implantable sustained release ciprofloxacin/carboxmethyl chitosan microspheres

Objective:To investigate the feasibility of implanting sustained release microspheres loaded with antibiotic in prevention and treatment of local infection after operation. Methods: The ciprofloxacin hydrochloride/carboxmethyl chitosan microspheres were prepared through emulsification and cross-linking process. A continuous flow release system was constructed to detect the in vitro releasing mechanism and bacteriostasis ability of microspheres. The in vivo release was detected using high performance liquid chromatography. The biodegradation and biocompatibility of microspheres were explored through transmission electron microscope and pathological findings. Results: The persisting release of ciprofloxacin was detected for 7 d in vitro and 10 d in vivo, which indicating the minimum inhibitory concentration still existing. No sudden release phenomenon was found during the whole process.Conclusion: The microspheres had excellent biocompatibility and releasing behavior and can be implanted for prevention and treatment of local infection after operation.