| 逆转录病毒介导的野生型p53基因转入对慢粒多药耐药细胞恶性表型的影响 |
| |
| 引用本文： | 陈学良,宋丽,刘春生,丛雅琴,裴玉丽,王应玞. 逆转录病毒介导的野生型p53基因转入对慢粒多药耐药细胞恶性表型的影响[J]. 山东大学学报(医学版), 1999, 0(4) |
| |
| 作者姓名： | 陈学良 宋丽 刘春生 丛雅琴 裴玉丽 王应玞 |
| |
| 作者单位： | 山东医科大学 |
| |
| 基金项目： | 卫生部科研基金!资助课题（96－2－153） |
| |
| 摘 要： | 以Ｋ５６２／ＨＨＴ 细胞为模型，采用重组逆转录病毒载体介导的基因转移技术，将人野生型ｐ５３ｃＤＮＡ转入该细胞系，研究外源性野生型ｐ５３ 基因在白血病多药耐药细胞表达的意义。结果显示，外源性野生型ｐ５３ 基因抑制细胞增殖，一般形态学观察见细胞明显向成熟型分化。流式细胞仪分析还显示ｐ５３ 基因诱导Ｋ５６２／ＨＨＴ细胞死亡且伴有细胞周期Ｇ１ 期的生长阻滞。结果表明，野生型ｐ５３ 基因对多药耐药的白血病细胞能部分降低其恶性表型
|
| 关 键 词： | p53基因 基因转移 逆转录病毒载体 K562/HHT细胞系 |
RETROVIRUS MEDIATED GENE TRANSFER OF WT p53 INTO K562/HHT CELL LINE REVERSES ITS MALIGNANT PHENOTYPE |
| |
| Chen Xueliang,Song Li,Liu Chunsheng,et al. RETROVIRUS MEDIATED GENE TRANSFER OF WT p53 INTO K562/HHT CELL LINE REVERSES ITS MALIGNANT PHENOTYPE[J]. Journal of Shandong University:Health Sciences, 1999, 0(4) |
| |
| Authors: | Chen Xueliang Song Li Liu Chunsheng et al |
| |
| Affiliation: | The Affilated Hospital of Shandong Medical University |
| |
| Abstract: | To study the significane of expression of exogenous wild type p53 gene(WP53G) in leukemic multidrug resistance cells, human WP53G cDNA was transferred by a recombinant retroviral vector in to K562/HHT cells. The Results suggest that exogenous WP53G inhibit cell growth and induce cell differentiation. Flow cytometric analysis showed that exogenous WP53G induced K562/HHT cells to die, which was accompanied by growth arrest in G 1 phase of the cell cycle. The conclusion can be drawn that the malignant phenotype can be at least partially reversed by restoring WP53G expression. |
| |
| Keywords: | p53 gene Gene transfer Retroviral vector K562/HHT cell line |
| 本文献已被 CNKI 等数据库收录！ |
