| 抗纤软肝颗粒对PDGF诱导下HSC细胞周期及胞内Ca2+浓度的影响 |
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| 引用本文： | 杨玲,程红球,朱清静,张赤志.抗纤软肝颗粒对PDGF诱导下HSC细胞周期及胞内Ca2+浓度的影响[J].中西医结合肝病杂志,2002,12(1):20-22. |
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| 作者姓名： | 杨玲 程红球 朱清静 张赤志 |
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| 作者单位： | 湖北中医学院附属医院肝病中心,武汉,430061 |
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| 基金项目： | 湖北省自然科学基金(课题编号:2000J042)，湖北省教育厅(课题编号:2000A06010) |
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| 摘 要： | 目的:研究抗纤软肝颗粒对血小板衍生生长因子(PDGF)诱导的肝星状细胞周期及胞内Ca~(2 )浓度的影响。方法:采用完全无血清培养,用流式细胞术分析抗纤软肝颗粒对PDGF诱导的HSC增殖的影响,应用Ca~(2 )荧光指示剂Fura-2/Am在荧光分光光度计上测定细胞内Ca~(2 )浓度。结果:与对照组相比,抗纤软肝颗粒处理组可显著地抑制PDGF诱导的HSC增殖及细胞内Ca~(2 )浓度的升高,尤以抗纤软肝颗粒5mg/ml组作用明显。结论:抗纤软肝颗粒能拮抗PDGF诱导的细胞内Ca~(2 )浓度的升高。
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| 关 键 词： | 血小板衍生生长因子 肝星状细胞 细胞内Ca~(2 ) 细胞周期 |
| 修稿时间： | 2001年12月4日 |
Effects of Kangxian Ruangan Granule on Platelet Derived Growth Factor (PDGF) Induced Cell Cycle and Intracellular Calcium Concentration of Hepatic Stellate Cells |
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| YANG Ling,CHENG Hongqiu,ZHU Qingjing,et al..Effects of Kangxian Ruangan Granule on Platelet Derived Growth Factor (PDGF) Induced Cell Cycle and Intracellular Calcium Concentration of Hepatic Stellate Cells[J].Chinese Journal of Integrated Traditonal and Western Medicine on Liver Diseases,2002,12(1):20-22. |
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| Authors: | YANG Ling CHENG Hongqiu ZHU Qingjing |
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| Institution: | YANG Ling,CHENG Hongqiu,ZHU Qingjing,et al. Liver Diseases Center of Affiliated Hospital of Hubei College of TCM |
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| Abstract: | Objective: To study the effect of Kangxinan Ruangan Granule (KXRGG) on platelet derived growth factor induced the cell cycle and intracellular calcium concentration. Methods: Flow cytometry and Calcium probe were used to analyse the proliferation and intracellular calcium concentration of HSC cultured in an absolutely serum free medium. Results s PDGF induced the proliferation of HSC and the increasing of intracellular calcium concentration were inhibited significantly in KXRGG treated group, especially in 5mg/ml KXRGG group as compared with control group. Conclusion: KXRGG inhibits the proliferation of HSC by influencing the intracellular concentration of calciam induced by PDGF. |
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| Keywords: | Platelet-derived Growth Factor Hepatic Stellate Cell Intracellular Calcium Cell Cycle |
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