结核分枝杆菌传播的分子流行病学特征

目的探讨结核分枝杆菌传播的基因型特征及其对于耐药结核菌近期传播的影响。方法选取13个社区作为监测点,应用分枝杆菌散在重复单位(MIRU)技术分析结核分枝杆菌DNA多态性。结果 558株结核分枝杆菌的12个MIRU位点检测产生143个基因型,其中成簇基因型66个,成簇率86.2%;74.6%的菌株属于北京家族,177个菌株(31.7%)属于山东基因型;耐多药菌株的近期感染估计值(46.7%)明显低于全敏感菌株(72.7%)。结论山东基因型菌株具有较强的在人群中传播的能力、较高的耐药性以及对抗督导化疗的能力;利用基因分型技术开展结核病监测可以有效促进结核病控制工作。

Molecular Epidemiological Characteristic Study on Transmission of Mycobacterium tuberculosis

OBJECTIVE To establish the molecular characteristics of Mycobacterium tuberculosis and the impact factor of recent transmission of drug resistant isolates.METHODS M.tuberculosis isolated from positive patients of 13 counties,were genotyped by Mycobacterial Interspersed Repetitive Units (MIRU) methods.RESULTS Twelve loci of MIRU were detected in 558 isolates,and a total of 143 MIRU patterns were confirmed.Seventyseven isolates had distinct patterns,and 481(86.2%)strains were in clusters.Shandong Cluster included 177 strains and 74.6% isolates belonged to Beijing family.The recent infection estimate value of multidrug-resistant strains was in lower level compared to the susceptible ones.CONCLUSIONS The Shandong cluster isolates have high capacity of person-to-person transmission,and a high level drug resistance;National TB Control Program should be further strengthened in genotyping survillence to address these problems.