| HPLC法测定更年灵胶囊中维生素B_1和维生素B_6的含量 |
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| 引用本文： | 陈锦容,胡敏.HPLC法测定更年灵胶囊中维生素B_1和维生素B_6的含量[J].海峡药学,2010,22(11):84-86. |
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| 作者姓名： | 陈锦容 胡敏 |
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| 作者单位： | 1. 福建同春药业股份有限公司,福州,350001
2. 福建省药品检验所,福州,350001 |
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| 摘 要： | 目的建立测定更年灵胶囊中维生素B1和维生素B6含量的方法。方法采用Hypersil BDS C18色谱柱（250×4.6mm,5μm）,甲醇-0.04%庚烷磺酸钠溶液（每100mL加0.3mL三乙胺,用磷酸调pH值至3.0）（20∶80）为流动相,检测波长275 nm。结果维生素B1和维生素B6的线性范围分别为12.1～602.8μg·mL-1（r=0.9999）和10.2～509.6μg·mL-1（r=0.9998）。平均加样回收率分别为为100.4%（RSD为1.8%,n=9）和98.3%（RSD为0.9%,n=9）。结论该法操作简便,结果准确,重复性好,可以用于更年灵胶囊的质量控制。
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| 关 键 词： | 更年灵胶囊 维生素B1 维生素B6 高效液相色谱法 |
Determination of Vitamin B1 and Vitamin B6 in GengNianLing Capsule by HPLC |
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| CHEN Jin-rong,HU min.Determination of Vitamin B1 and Vitamin B6 in GengNianLing Capsule by HPLC[J].Strait Pharmaceutical Journal,2010,22(11):84-86. |
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| Authors: | CHEN Jin-rong HU min |
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| Institution: | 1.Fujian TongChun pharmaceutical CO.,Lid.,Fuzhou 350001,China;2.Fujian institute for drug control,Fuzhou,350001,China) |
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| Abstract: | OBJECTIVE To establish a method for determination of Vitamin B1 and Vitamin B6 in GengNianLing Capsule.METHODS The Hypersil BDS C18 column（250mm×4.6mm,5μm）was used and the mobile phase was 1-heptanesulfonic acid sodium salt solution（20∶80）.The detection wavelength was 275nm.RESULTS The linear rangs of Vitamin B1 were 12.1～602.8μg·mL-1（r=0.9999）and Vitamin B6,10.2～509.6μg·mL-1（r=0.9998）,The average recovey of Vitamin B1 was 100.4% with RSD=1.8%（n=9）.The average recovey of Vitamin B6 was 98.3% with RSD=0.9%（n=9）.CONCLUSION The method is simple and accurate with good reproducibility and can be used for the quality control of GengNianLing Capsule. |
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| Keywords: | GengNianLing Capsule Vitamin B1 Vitamin B6 HPLC |
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