| 用PCR检测微小病毒B19对儿童急性白血病发病的影响 |
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| 引用本文： | 王玉龙,张惠明.用PCR检测微小病毒B19对儿童急性白血病发病的影响[J].临床儿科杂志,1998,16(5):294-296. |
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| 作者姓名： | 王玉龙 张惠明 |
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| 作者单位： | 上海第二医科大学附属新华医院儿科，上海第二医科大学生化教研室分子生物学实验室，上海第二医科大学附属新华医院儿科，上海第二医科大学附属新华医院儿科，上海第二医科大学附属新华医院儿科，上海第二医科大学附属新华医院儿科 200092，200092，200092，200092，200092 |
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| 摘 要： | 应用１％ＮＰ－４０处理骨髓涂片，套式ＰＣＲ扩增技术对２８例儿童急性白血病者进行微小病毒Ｂ１９ＤＮＡ检测，其中ＡＬＬ１５例，ＡＭＬ１３例。并对阳性者同时采用免疫表型检测，结果显示５例阳性，且均为ＡＬＬ患者。
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| 关 键 词： | 急性白血病 微小病毒B19 PCR 骨髓细胞涂片 儿童 |
Stady on the influence of parvovlrus B19 detected by PCR on pathogenesis of childhood acute leukemia. |
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| Wang yu-long,Zhang Hui-ming,Gu Long-jun,Liang Hui,Zhao Hui-jun,Gu Mei-yu.Stady on the influence of parvovlrus B19 detected by PCR on pathogenesis of childhood acute leukemia.[J].The Journal of Clinical Pediatrics,1998,16(5):294-296. |
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| Authors: | Wang yu-long Zhang Hui-ming Gu Long-jun Liang Hui Zhao Hui-jun Gu Mei-yu |
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| Institution: | Wang yu-long,Zhang Hui-ming,Gu Long-jun,Liang Hui,Zhao Hui-jun,Gu Mei-yu,Department of Pediatrics,Xin-hua Hospital Affiliated to Shanghai Second Medical University,Shanghai 200092 |
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| Abstract: | Using 1% NP-40 treated bone marrow smear and nested PCR skills, parvovirus B19-DNA were detected in 28 children with acute leukemia(15 cases with ALL and 13 cases with AMD. Immuno-phenotyping assay would be un- dertaken if there was a positive B19-DNA. Showing that five positive B 19 DNA cases all were ALL. Three cases were B-ALL and two cases T-ALL by immuno-phenotyping analysis. Also these five cases belonged to L2 type by FAB classification. Indicating that parvovirus B19 may exert influence on the pathogenesis of childhood acute lymphocytic leukemia. The nested PCR on bone marrow smear is a simple, specific and sensitive method in detecting parvovirus Bl 9-DNA directly for clinical diagnosis of B19 infection. |
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| Keywords: | leukemia parvovirusB19 PCR bone marrow smear |
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