| 氟对大鼠肝功及GSH-Px基因表达影响 |
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| 引用本文： | 刘连,徐园园,许洁,黄厚今.氟对大鼠肝功及GSH-Px基因表达影响[J].中国公共卫生,2010,26(5). |
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| 作者姓名： | 刘连 徐园园 许洁 黄厚今 |
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| 作者单位： | 1. 遵义医学院组织学与胚胎学教研室,遵义,563003
2. 遵义医学院预防医学教研室 |
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| 基金项目： | 贵州省科学技术基金，贵州省省长基金 |
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| 摘 要： | 目的探讨不同浓度氟对大鼠肝功和谷胱苷肽过氧化物酶(GSH-Px)基因表达及肝细胞超微结构影响。方法在饮水中加入不同剂量氟化钠喂饲大鼠30 d后,测定血清氟含量、门冬氨酸氨基转移酶(AST)、丙氨酸氨基转移酶(ALT)活力、肝组织中GSH-Px活性及mRNA表达,观察大鼠肝细胞超微结构。结果随染氟剂量增加,各剂量组大鼠血氟浓度逐渐增加,血清中AST、ALT含量逐渐增加,呈剂量-效应关系(R=0.889,R=0.729,P0.01);GSH-Px酶活力单位从(19.672±1.025)下降至(11.777±1.529),与对照组比较,差异有统计学意义(P0.01);GSH-Px的mRNA表达下降(P0.05)。中、高剂量组大鼠肝细胞超微结构可见线粒体肿胀,有较多脂肪变性。结论过量氟可能诱导GSH-Px的mRNA表达下降,使大鼠肝脏抗氧化系统受到抑制。
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| 关 键 词： | 氟 肝细胞超微结构 谷胱甘肽过氧化物酶 基因表达 |
Effect of fluoride on liver function,expression of GSH-Px gene and ultrastructure of liver cell in rats |
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| LIU Lian,XU Yuan-yuan,XU Jie,et al..Effect of fluoride on liver function,expression of GSH-Px gene and ultrastructure of liver cell in rats[J].Chinese Journal of Public Health,2010,26(5). |
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| Authors: | LIU Lian XU Yuan-yuan XU Jie |
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| Institution: | LIU Lian,XU Yuan-yuan,XU Jie,et al.Department of Histology , Embryology,Zunyi Medical College(Zunyi 563003,China) |
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| Abstract: | Objective To study the effects of different dosages of fluoride on liver function,expression of GSH-Px gene,and the ultrastructure of liver cell in rats.Methods Drinking water with various concentrations of sodium fluoride was given to rats for 1 month.The contents of the fluoride,the activities of asparate aminotransferase(AST),alamine aminotransferase(ALT),glutathion peroxidase(GSH-Px),and mRNA of GSH-Px in liver of the rats were measured.Results The serum fluoride increased in dose-effect manner with the... |
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| Keywords: | fluoride hepatic cell ultrastructure GSH-Px gene expression |
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