First isolation of Japanese encephalitis from Culex quinquefasciatus in Thailand |
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Authors: | Nitatpattana Narong Apiwathnasorn Chamnarn Barbazan Philippe Leemingsawat Somjai Yoksan Sutee Gonzalez Jean-Paul |
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Institution: | Center for Vaccine Development, Research Center for Emerging Viral Diseases, Institute of Science and Technology for Research and Development, Mahidol University, Nakhon Pathom, Thailand. |
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Abstract: | Isolation of Japanese encephalitis (JE) virus using C6/36 cell and immunofluorescence virus antigen detection techniques was attempted from female mosquitoes collected with CDC gravid traps in Samut Songkhram Province in the central region and in Phuket Province in southern Thailand, in 2003. One thousand and eighty female mosquitoes including 6 species of the Culicidae family (Culex quinquefasciatus, Cx. gelidus, Cx. tritaeniorhynchus, Cx. whitmorei, Cx. vishnui complex, Cx. s.g. culiciomyia) (pooled by specific specimen), were processed for virus isolation. Two pools of Cx. quinquefasciatus yielded a JE virus isolation. This represents the first report of JE virus isolation from Cx. quinquefasciatus in Thailand. |
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