甘草甜素及地塞米松对体外培养足细胞增殖的影响研究

目的通过建立嘌呤霉素(PAN)诱导小鼠足细胞MPC5损伤模型,观察甘草甜素(GL)及地塞米松(DEX)对足细胞及受损足细胞增殖的影响作用,探讨GL在体外培养足细胞损伤中是否具有类似DEX的防护作用。方法体外培养小鼠足细胞,分为对照组、PAN组、GL组、DEX组、PAN+GL组、PAN+DEX组。对照组采用RPMI-1640培养液培养;PAN组加入终浓度50mg/LPAN;GL组加入不同浓度的GL,终浓度分别为50、100、200、400、600、800mg/L;DEX组加入终浓度1μmol/LDEX;PAN+GL组同时加入PAN(终浓度50mg/L)和GL(终浓度分别为50、100、200、400、600、800mg/L);PAN+DEX组同时加入PAN(终浓度50mg/L)和DEX(终浓度1μmol/L),培养24h及48h,采用MTT检测细胞的增殖活性。结果与对照组相比,PAN组24h及48h时足细胞增殖的光密度(A)值均明显降低(P<0.01),抑制率(IE)分别为55.56%、59.10%;GL组中不同浓度组24h时A值较对照组均明显降低(P<0.01),48h时GL50mg/L组A值与对照组比较差异无统计学意义(P>0.05),其余各组A值较对照组均明显降低(P<0.01);低浓度GL组(100～400mg/L)对足细胞的IE48h较24h明显下降,高浓度GL组(600～800mg/L)对足细胞的IE48h较24h明显升高。DEX组24、48h足细胞增殖A值较对照组均明显降低(P<0.01),48h时足细胞的IE较24h时差异无统计学意义(P>0.05)。PAN+GL组50～600mg/L浓度范围内,24h、48h时足细胞增殖A值均呈剂量依赖性升高(P<0.01),但PAN+GL组800mg/L浓度时足细胞增殖活性不再增加。PAN+DEX组24、48h时足细胞增殖A值均明显升高(P<0.01)。结论 PAN能显著抑制足细胞增殖,GL及DEX对体外培养的正常足细胞增殖有轻微抑制作用,但二者均能促进受损足细胞的增殖,且GL在一定范围内呈剂量依赖性关系,对PAN诱导足细胞损伤具有类似DEX的防护作用。

The Vitro Study on Intervention of Glycyrrhizin and Dexamethasone in Podocytes Proliferation

Objective To establish podocyte injury model induced by puromycin aminonucleoside （PAN）, observing the impact of glycyrrhizin （GL） and dexamethasone （DEX） on proliferation of normal podoeyte and injured podocyte, and exploring whither GL was similar protective effect to DEX on podocyte injury in vitro. Methods Murine podocytes were divided into 6 groups： control group （apply RPMI - 1640 at same quantity）, PAN group （PAN 50mg/L）, GL group （ GL50, 100, 200, 400, 600, 800mg/L）, DEX group （DEX 1μmol/L）, PAN ＋ GL group （PAN 50mg/L ＋ GL50, 100, 200, 400, 600, 800mg/L） , PAN ＋ DEX group （ PAN 50mg/L ＋ DEX 11mol/L）. the proliferative podoeyte cells were detected with MTr assay-after 24h or 48h treatment. Results Compared to control group, the proliferation of podocyte （A value） in PAN group was significantly lower 2dh and 48h （P 〈0. 01）, the proliferation inhibition rate was 55.56% and 59. 10% ; The A value in GL group was significantly lower 24h and 48h, except that the A value in GLS0mg/L group was not decreased after 48h treatment compared to control group （P 〉 0. 05 ） ; the inhibition rate in lower dose of GL （ 100 - 400 mg/L） 48h was decreased comparable to 24h while it remain high level in higher dose of GL （600 -800 rag/L） 48h. The A value in DEX group was significantly lower 24h and d8h （P 〈0.01 ）, the inhibition rate was almost similar between 2dh and 48h. Compared to PAN group, the A value in PAN GL 50 -600mg,/L groups were significantly increased in a dose - dependent manner after 24h and 48h treatment （P 〈 0.01 ）, but the ability of podocyte proliferation was not strengthened with high dose （ PAN ＋ GL 800mg/L） group; The A value in PAN ＋ DEX group was significantly enhanced both 24h and 48h （P 〈 0.01 ）. Conclusion PAN can inhibit podocyte proliferation severely, GL and DEX have slightly suppressive effects on podocyte proliferation, but both of them can improve proliferation of injured podocyte induced by PAN, while GL has same cytoprotective effect of DEX on injured podocyte cultured in vitro.