| 人骨髓间充质干细胞体外培养及生物学特性观察 |
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| 引用本文： | 法宪恩,王利霞,侯剑峰,张瑞成,王海永,杨辰垣.人骨髓间充质干细胞体外培养及生物学特性观察[J].郑州大学学报(医学版),2005,40(3):445-447. |
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| 作者姓名： | 法宪恩 王利霞 侯剑峰 张瑞成 王海永 杨辰垣 |
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| 作者单位： | 华中科技大学同济医学院协和医院心血管外科,武汉,430022;郑州大学第二附属医院心外科,郑州,450014;郑州大学第二附属医院心外科,郑州,450014;华中科技大学同济医学院协和医院心血管外科,武汉,430022 |
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| 基金项目： | 河南省科技创新人才工程资助项目2003043 |
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| 摘 要： | 目的：观察体外培养的人骨髓间充质干细胞(MSCs)部分生物学特性。方法：自健康人骨髓中分离出MSCs，进行原代培养，第5d首次更换培养液，以去除未贴壁细胞，以后每周换液2次，细胞铺满整个培养瓶底90％时开始传代培养。倒置相差显微镜下观察原代及传代细胞形态及生长特性，并绘制传代细胞的生长曲线。流式细胞仪检测第3代MSCs细胞表面分子CD29、CD44，以进行MSCs鉴定。结果：MSCs体外培养为贴壁生长。MSCs原代培养约18～21d才达传代要求；传代培养细胞生长潜伏期仅24～36h，对数增长期约3～5d，之后为平台期，4～6d传1代。传至第8代，出现凋亡征象。第3代MSCs细胞表面CD29、CD44的表达率分别为90．6％和91．5％，MSCs均一性较高。结论：从人骨髓中分离出的MSCs在体外环境中具有很强的自我更新和分裂增殖能力。
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| 关 键 词： | 间充质干细胞 骨髓 细胞培养 人 生物学特性 |
| 修稿时间： | 2004年9月21日 |
Biologic characteristics of human bone marrow-derived mesenchymal stem cells cultured in vitro |
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| FA Xian'en,WANG Lixia,Hou Jianfeng,ZHANG Ruicheng,Wang Haiyong,YANG Chenyuan.Biologic characteristics of human bone marrow-derived mesenchymal stem cells cultured in vitro[J].Journal of Zhengzhou University: Med Sci,2005,40(3):445-447. |
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| Authors: | FA Xian'en WANG Lixia Hou Jianfeng ZHANG Ruicheng Wang Haiyong YANG Chenyuan |
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| Institution: | FA Xian' en 1,2),WANG Lixia 2),HOU Jianfeng 2),ZHANG Ruicheng 2),WANG Haiyong 2),YANG Chenyuan 1) 1)Department of Cardiovascuology,the Union Hospital,Tongji Medical College,Huazhong Science and Technology University,Wuhan 430022 2)Department of Cardiac Surgery,the Second Affiliated Hospital,Zhengzhou University,Zhengzhou 450014 |
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| Abstract: | Aim: To investigate the biological characteristics of human bone marrow-derived mesenchymal stem cells (MSCs) in vitro. Methods: Human MSCs were isolated from bone marrow and purified by centrifuge and cultured in vitro. The proliferation and growth characteristics of MSCs were observed in primary and passage culture. Results: Human bone marrow-derived MSCs showed active proliferation capacity in vitro in primary and passage cultures.Conclusion: MSCs derived from human bone marrow have been successfully cultured. It founds a base for further investigation and using of mesenchyaml stem cells. |
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| Keywords: | mesenchymal stem cell bone marrow cell culture human biologic characteristic |
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