IL-27对哮喘小鼠血清IgE的影响

目的研究IL-27对卵白蛋白（OVA）激发哮喘小鼠血清IgE的影响。方法 24 只雌性BALB/c小鼠随机分为生理盐水组、哮喘组及IL-27组，每组8只。应用OVA建立哮喘模型，IL-27组小鼠应用1 μg IL-27（溶于 50 μl PBS中）滴鼻给药，ELISA法测定小鼠血清IL-4和 IFN-γ 浓度，荧光酶标法测定小鼠血清总IgE和OVA特异性IgE浓度；计数支气管肺泡灌洗液（BALF）中嗜酸粒细胞数量。结果 IL-27 组小鼠血清IL-4浓度为（45.6±10.1）μg/L，明显低于哮喘组的（79.3±14.8）μg/L（P 〈 0.05）；IL-27 组小鼠血清IFN-γ浓度为（63.8±11.5）μg/L，明显高于哮喘组的（25.7±8.2）μg/L（P 〈 0.05）；IL-27组小鼠血清总IgE和OVA特异性IgE浓度分别为（2.77±0.51）KU/L、（0.48±0.09）KU/L，均明显低于哮喘组的（5.88±0.86）KU/L、（1.89±0.15）KU/L（P均〈0.05）；IL-27组小鼠 BALF中嗜酸粒细胞数量为（2.21±0.33）×107/L，明显低于哮喘组的（12.82 ± 2.17）×107/L（P 〈 0.01）。结论 IL-27可能通过促进IFN-γ合成、抑制IL-4合成，降低哮喘小鼠血清总IgE和OVA特异性IgE浓度，降低BALF中嗜酸粒细胞数量。

The Influence of IL-27 on Serum IgE in Ovalbumin-induced Asthmatic mice

Objective To investigate the influence of IL--27 on serum IgE of asthmatic mice challenged by ovalbumin （OVA）. Methods 24 female BALB/c mice were randomly divided into physiological saline group, asthmatic group and IL--27 group with each group of eight ones. The mice of IL--27 group were administered with 1μg intranasal IL--27（dissolved in 50 μL PBS） after the establishment of asthmatic model with the application of OVA. The serum IL - 4 and IFN-- 7 concentration of mice were detected with ELISA, the total serum IgE and OVA specific IgE concentration of mice were detected with fluorescence enzyme linked immunosorbent assay and the number of eosinophil granulocytes in bronchoalveolar lavage fluid （BALF） was counted. Results The serum IL--4 concentration （45.6±10.1） μg/L] of mice in IL--27 group was significantly lower than that of asthmatic group （79.3±14.8）μg/L] （P〈0.05）. The serum IFN--7 concentration （63.8±11.5） μg/L]of mice in IL--27 group was obviously higher than that of the asthmatic group （25.7±8.2）μg/L]（P〈0.05）. The total serum IgE （2.77±0.51） KU/L] and OVA specific IgE concentration （0. 48±0. 09） KU/L]of the mice in IL--27group were significantly lower than those （5.88±0.86） KU/L, （1.89±0.15）KU/L] of the asthmatic group （both P〈.05）. The BALF eosinophil granulocytes counts （2.21±0.33））〈 107/L]of the mice in IL--27 group was significantly lower than that of the asthmatic group （12.82±2.17） × 107/L] （P〈0. 01）. Conclusion IL--27 reduces total serum IgE and OVA specific IgE concentration, the number of eosinophil granulocytes in BALF of asthmatic mice through promoting IFN--7 synthesis and inhibits IL--4 synthesis.