肝细胞色素p450 1A1在大鼠非酒精性脂肪肝形成中的作用

目的研究肝细胞色素p450 1A1在非酒精性脂肪肝大鼠形成中的作用.方法Wistar大鼠40只,随机分为正常对照组和高脂饲料2、4、8和12周组(其中每组各8只);HE染色光镜观察肝脏组织病理改变;硫代巴比妥酸法测定肝脏组织丙二醛(MDA)的含量变化;免疫组织化学和Western blot方法研究高脂饲料诱导的大鼠脂肪肝形成中肝细胞色素p450 1A1表达变化.结果高脂饲料组大鼠肝脏内MDA含量明显高于对照组,随着脂肪肝程度的加重,MDA含量逐渐增强,肝细胞色素p450 1A1蛋白表达亦明显增强.结论非酒精性脂肪肝大鼠肝细胞色素p450 1A1表达变化与脂肪肝引起的脂质过氧化损伤程度密切相关.

Effect of hepatocyte cytochrome p450 1A1 in nonalcoholic steatosis rats

Objective To study the effect of hepatocyte cytochrome p450 1A1 expression in rat nonalcoholic steatosis model.Methods Fourty Wistar rats were randomly divided into control group and high-fat diet group, and high-fat diet group was divided into 4 subgroups (2,4,8 and 12weeks). Each subgroup had 8 rats.The expression of hepatocyte cytochrome p450 1A1 antigen in rat nonalcoholic steatosis model induced by high fat diet was detected with immohistochemistry and Western blot.Meanwhile, levels of malondialdehyde (MDA) in liver tissue were detected by using thio-barbituric acid.Results Levels of MDA in liver tissue and the expression of hepatocyte cytochrome p450 1A1 antigen in rat nonalcoholic steatosis model induced by high fat diet were significantly increased than those in normal . There were significant positive correlation between those and degree of nonalcoholic steatosis.Conclusion Nonalcoholic steatosis induced liver damage was associated with a generalized induction of hepatocyte cytochrome p450 1A1 and lipid peroxidation.