高速逆流色谱法分离纯化银杏叶中白果内酯和银杏内酯A、B、C

目的从银杏叶中分离纯化白果内酯和银杏内酯A、B、C。方法银杏叶提取液经醋酸乙酯萃取、D-101大孔吸附树脂柱和Al2O3柱纯化后得到总内酯提取物,提取物再经两次高速逆流色谱分离制备4种内酯单体。结果25%乙醇热提取、醋酸乙酯萃取、D-101柱、pH4.0的Al2O3柱对总内酯的提纯最终使总内酯质量分数达到44.98%。经高速逆流色谱制备得到的白果内酯和银杏内酯A、B、C最高质量分数分别为98.3%、98.9%、98.8%、98.4%。结论本法简便快速、回收率高,为银杏叶中内酯单体的制备提供了一种新方法

Separation and purification of bilobalide and ginkgolides A, B, and C from Ginkgo biloba leaves by high-speed counter-current chromatography

Objective To separate and purify bilobalide and ginkgolide A,B,and C from Ginkgo biloba leaves. Methods Firstly the extract of G. biloba leaves was purified by extraction of acetic ether,D-101 macroporous resin,and Al2O3 (pH 4) to obtain the crude extract of lactones. Then the crude extract was further purified by high-speed counter-current chromatography(HSCCC) to prepare the monomer of bilobalide and ginkgolide A,B,and C. Results The crude extract of lactones with the purity of 44.98% was obtained by extraction with 25% alcohol,acetic ether,D-101 macroporous resin,and Al2O3 (pH 4).When the crude extract was further purified by HSCCC,the different purity of bilobalide and ginkgolide A,B,and C was got. The maximal purity of bilobalide and ginkgolide A,B,and C was 98.3%,98.9%,98.8%,and 98.4%. Conclusion This method,which is simple and rapid,provides a new way to separate and purify the lactones from G. biloba leaves.