| Abstract: | Bilateral vasectomy was performed in 10 adult dogs, using another set of 10 as a matching control. Both normal testes were removed from 1 vasectomized and 1 normal dog at weekly intervals. The distribution of alkaline phosphatase, glycogen, lipids, and cholesterol was studied histochemically. The epididymis was also studied. Marked degeneration occurred in the seminiferous tubules, and spermatogenesis ceased. Alkaline phosphatase and lipids decreased appreciably, but the basal cells (spermatogonia) remained practically unaffected. There was thickening of the PAS positive, diastase resistant tunica propria from the fourth week on. The lydig cell clusters became more prominent and showed increase in cholesterol and lipids. The epididymis appeared distended but without any visible sperms. The epithelial cells were markedly flattened. Alkaline phosphatase decreased near the lumal border, but the nuclei, now packed at the periphery, gave a more intense reaction. The functional significance of these changes, particularly in view of the large scale use of vasectomy today, is discussed. |
