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A new leukemia cell line with Philadelphia chromosome characterized as basophil precursors
Authors:K Kishi
Affiliation:The First Department of Internal Medicine, Niigata University, School of Medicine, Niigata, Japan
Abstract:A new myeloid cell line (KU812) was established from a patient with blastic crisis of chronic myelogenous leukemia. His blasts were morphologically characteristic of immature basophils and basophil colonies were grown in agar culture of the blood mononuclear cells. Suspension culture of his blood cells was continued for more than 2.5 years. The KU812 cells morphologically showed a fine reticular nuclei with nucleoli, and some of them contained metachromatic granules with toluidine blue (TB) staining. These granules were positive for astra blue (AB) staining. Immunological marker studies revealed that there were no lymphoid characters except Fc receptors. The KU812 cells grew colonies in in vitro agar cultures, which were proved to be composed of basophils by TB staining and AB staining. Cytogenetical analysis showed marked aneuploidy and was positive for the Philadelphia chromosome (Ph1). The cell lysate was proved to contain histamine. These data suggest that KU812 is a cell line from leukemic basophil precursors. This is the first human basophil cell line, and KU812 will be useful in clarifying the mechanism of basophilic differentiation of the stem cells.
Keywords:Chronic myelogenous leukemia  blastic crisis  basophils  leukemia cell line  cell differentiation  astra blue staining  astra blue  toluidine blue  glucose-6-phosphate dehydrogenase  chronic myelogenous leukemia  the Philadelphia chromosome  mononuclear cells(s)  fetal bovine serum  human placental conditioned medium  myeloperoxydase  naphthol ASD chloroacetate esterase  alpha-naphtyl butyrate esterase  alkaline phosphatase  acid phosphatase  periodic acid Schiff  Sudan black B  Epstein-Barr virus nuclear antigen  terminal deoxyribonucleotidyl transferase  granulocyte/macrophage colony  phosphate buffered saline
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