Stanford A型主动脉夹层差异表达基因生物学通路分析

 目的  通过对急性Stanford A型主动脉夹层患者主动脉组织差异表达基因的通路分析,探讨主动脉夹层的发病机制。方法  应用人类全基因组表达谱微阵列芯片检测急性Stanford A型主动脉夹层患者与器官捐献者主动脉组织的基因表达,GenomeStudio Gene Expression Module v1.0软件进行数据结果分析。采用荧光定量real time PCR验证芯片结果。以KEGG数据库对差异表达基因进行通路分析。结果  筛选出差异表达基因数1 309个,real-time PCR验证结果与芯片检测结果一致。通路分析发现两组之间黏着斑通路的基因表达发生显著变化,同时血管平滑肌收缩通路和肌动蛋白细胞骨架调节通路的基因表达也有显著差异。结论  差异基因通过黏着斑通路、血管平滑肌收缩通路和肌动蛋白细胞骨架调节通路相互作用,可能在急性Stanford A型主动脉夹层的发病机制中发挥了重要作用。

Pathway analysis of differentially expressed genes in patients with acute Stanford type A aortic dissection

Objective  To study the pathogenesis of aortic dissection by pathway analysis of differentially expressed genes in patients with acute Stanford type A aortic dissection.Methods  Ascending aorta specimens from patients with acute Stanford type A dissection were taken during surgery,and compared with those from normal ascending aorta of organ donors using the gene expression microarray.Data were analyzed with GenomeStudio Gene Expression Module v1.0.Validation of microarray gene expression was performed with quantitative real-time PCR.All differentially expressed genes were submitted for pathway analysis using KEGG database.Results  According to our definition,1 309 genes were differently expressed in dissected and control aorta specimens.The result of quantitative real-time PCR was consistent with that of microarray.Compared with the control group,patients with aortic dissection had significantly different gene-expression pathways involving focal adhesion,vascular smooth muscle contraction and regulation of stanford type A actin cytoskeleton.Conclusions  Genes with different expression might have interacted through focal adhesion,vascular smooth muscle contraction and regulation of actin cytoskeleton pathways and played a contributory role in the pathogenesis of aortic dissection.