生物样品中抗凝血类杀鼠剂分析研究进展

 在我国,抗凝血类杀鼠剂中毒频繁发生,易被误诊。检测生物样品中抗凝血类杀鼠剂对临床诊断、治疗和法医学调查有重要意义。本文综述了近年文献报道的生物样品中抗凝血类杀鼠剂的分析方法,着重比较各种生物样品选择、样品前处理、分离检测技术,并结合本课题组的应用案例给出血液、头发分析的结果参考。血液适用于抗凝血类杀鼠剂分析,其主要前处理方法有液 液提取、蛋白质沉淀等。头发可作为血液样品的重要补充,用于摄药历史的法医学调查。液相色谱 质谱(liquid chromatography mass spectrometry,LC/MS)检测法兼顾灵敏度与选择性,是目前最常用的分析生物样品中抗凝血类杀鼠剂的技术。

Review of analysis on anticoagulant rodenticides in biological specimens

In China,poisoning of anticoagulant rodenticides is common,and the diagnosis is difficult.Analysis of anticoagulant rodenticides in biological samples is very important to clinical diagnosis,treatment,and forensic investigation.The present article reviews the recently reported analytical methods for anticoagulant rodenticides in biological specimens,focusing on the choice of biological specimen,sample preparation,separation and detection technologies,along with the application to poisoning cases using blood or scalp hair by the the authors′ study group.The conclusions:blood is appropriate for analysis of anticoagulant rodenticides,and the sample preparation mainly includes liquid liquid extraction or protein precipitation.Hair could serve as implementation to blood in the forensic investigation of exposure history.To date,liquid chromatography/mass spectrometry(LC/MS) is the commonest technology for analyzing anticoagulant rodenticides in biological specimens for its sensitivity and selectivity.