Poliovirus replicons encoding the B subunit of Helicobacter pylori urease protect mice against H. pylori infection |
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Authors: | Smythies Lesley E Novak Miroslav J Waites Ken B Lindsey J Russell Morrow Casey D Smith Phillip D |
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Affiliation: | Department of Medicine (Gastroenterology), University of Alabama at Birmingham, ZRB 633, 703 19th Street South, Birmingham, AL 35294, USA. |
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Abstract: | We developed a novel vaccine for Helicobacter pylori based on a poliovirus vector in which capsid genes were replaced with the gene for the B subunit of H. pylori urease (UreB). Mice were vaccinated with UreB or control (L1) replicon and challenged with H. pylori. Twenty percent of mice vaccinated prophylactically with UreB, but 80% vaccinated with L1, and then challenged with H. pylori became infected (P = 0.003). Seventy-three percent of mice with established H. pylori infection vaccinated therapeutically with UreB replicon cleared their infection compared to 33% vaccinated with L1 (P = 0.067). In therapeutically vaccinated mice with residual infection, UreB-vaccinated animals had fewer H. pylori than L1-vaccinated mice (P < 0.05). Anti-urease antibody titres in prophylactically, but not therapeutically, vaccinated mice were markedly higher in animals that received UreB versus L1 replicon (P = 0.01). Vaccination with poliovirus vector containing the gene for the B subunit of H. pylori urease provides significant prophylactic and strong therapeutic protection against H. pylori in mice. |
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