On-line immunoaffinity extraction and HPLC analysis of flunitrazepam and its main metabolites in serum |
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Authors: | Deinl I Franzelius C Angermaier L Mahr G Machbert G |
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Institution: | Institute of Forensic Medicine, Erlangen, Germany. |
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Abstract: | A sensitive, simple, and rapid method for the determination of flunitrazepam and its major metabolites (7-aminoflunitrazepam, 7-acetamidoflunitrazepam, and norflunitrazepam) in serum and plasma is presented. The on-line procedure uses an immobilized, highly reusable antibody against benzodiazepines for selective extraction from serum followed by analysis by high-performance liquid chromatography with ultraviolet detection. This reliable method provides a limit of detection of 1 ng/mL serum, and results are obtained in less than 40 min. |
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