| Tenuigenin对转染APP695cDNA的SH-SY5Y细胞β-淀粉样蛋白生成的抑制作用及机制 |
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| 引用本文： | 贾竑晓,马辛,姜勇,阮燕,张继志,涂鹏飞,张岱. Tenuigenin对转染APP695cDNA的SH-SY5Y细胞β-淀粉样蛋白生成的抑制作用及机制[J]. 首都医科大学学报, 2005, 26(4): 428-431 |
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| 作者姓名： | 贾竑晓 马辛 姜勇 阮燕 张继志 涂鹏飞 张岱 |
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| 作者单位： | 首都医科大学附属北京安定医院;北京大学精神卫生研究所;首都医科大学附属北京安定医院;北京大学药学院;北京大学精神卫生研究所 |
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| 基金项目： | 国家自然科学科学基金(39870276,39840010)，国家重大基础项目研究基金(G1999064007)，北京市中医药管理局科研基金(JJ99-26)资助项目 |
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| 摘 要： | 目的用转染APP695cDNA的SH-SY5Y细胞模型,研究tenuigenin对β-淀粉样蛋白(Aβ)生成的抑制作用及其作用机制。方法应用免疫沉淀和蛋白印迹法(Westernblot)检测Aβ含量,应用MTT和LDH法测定tenuigenin的细胞毒性。结果Tenuigenin降低SH-SY5YAPP695细胞Aβ分泌水平;无细胞增生和毒性作用;能抑制SH-SY5YAPP695细胞APPC99的生成,但对SH-SY5YSPA4CT细胞APPC99生成无抑制作用,亦不影响该细胞株Aβ的分泌。结论Tenuigenin可能通过抑制β-分泌酶对APP的水解,降低SH-SY5YAPP695细胞Aβ水平。Tenuigenin这一针对阿尔茨海默病(Alzheimerdisease,AD)上游病理环节的药效,显示了其在AD治疗方面重要的开发和临床应用的价值,值得进一步研究。
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| 关 键 词： | 阿尔茨海默病 β-淀粉样蛋白 C99 免疫沉淀Western印迹法 |
| 收稿时间： | 2005-06-30 |
| 修稿时间： | 2005-06-30 |
Inhibitory Effect of Tenuigenin on Beta Amyloid Protein Production of SH-SY5Y Cells Transfected with APP695 cDNA |
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| Jia Hongxiao ,,Ma Xin ,Jiang Yong ,Ruan Yan ,Zhang Jizhi ,Tu Pengfei ,Zhang Dai. Inhibitory Effect of Tenuigenin on Beta Amyloid Protein Production of SH-SY5Y Cells Transfected with APP695 cDNA[J]. Journal of Capital Medical University, 2005, 26(4): 428-431 |
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| Authors: | Jia Hongxiao Ma Xin Jiang Yong Ruan Yan Zhang Jizhi Tu Pengfei Zhang Dai |
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| Affiliation: | Jia Hongxiao 1,3,Ma Xin 1,Jiang Yong 2,Ruan Yan 3,Zhang Jizhi 1,Tu Pengfei 2,Zhang Dai 3* |
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| Abstract: | Objective To investigate the inhibitory effect of tenuigenin on beta amyloid protein(Aβ) which caused Alzheimer's Disease(AD).Methods Western blotting was used to test the Aβ and C99 levels of cells; MTT and LDH was used to test the neurotoxicity of tenuigenin to the cells.Results Tenuigenin can inhibit the production of beta amyloid protein of SH-SY5Y APP695 cells, didn't have neurotoxicity to cells; it can decrease Aβ and C99 levels of SH-SY5Y APP695 cells, but it didn't decrease Aβ and C99 levels of SH-SY5Y SPA4CT cells.Conclusion Tenuigenin can inhibit the production of beta amyloid protein of SH-SY5Y APP695 cells, it's mechanism may come through affecting the β-secretase proteolytic activities. |
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| Keywords: | Alzheimer disease(AD) amyloid β-peptides(Aβ) C99 Western blotting |
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