胰腺十二指肠同源盒在2型糖尿病大鼠胰腺组织中的表达及罗格列酮的干预作用

目的观察胰腺十二指肠同源盒（PDX-1）在T2DM大鼠胰腺组织中的表达及罗格列酮（RGZ）干预后的变化。方法以链脲佐菌素腹腔注射建立T2DM大鼠模型，HE染色和免疫组化对胰腺进行形态学观察，RT-PCR测定胰岛素和PDX-1 mRNA，Western blot测定PDX-1蛋白的表达。结果DM大鼠胰腺胰岛素染色阳性面积（12．75±2．18）、PDX-1平均光密度（O．240±0．051）、PDX-1 mRNA（0．153±0．071）及蛋白（O．253±0．028）的表达均显著低于正常对照组（42．61±2．68，0．648±0．087，0．49±0．032，0．720±0．036）（P均〈0．01），RGZ干预后较DM组均显著增加（20．67±2．14，0．460±0．045，0．370±0．029，0．59±0．050）（P〈0．05～0．01）。结论DM大鼠胰岛B细胞功能受损，PDX-1的表达明显下降，RGZ治疗可改善胰岛B细胞功能，并使PDX-1的表达上调。

The expression of the pancreatic duodenal homeobox-1(PDX-1) in pancreas of experimental type 2 diabetic rat and the effect of rosiglitazone on its expression

Objective To observe PDX-1 expression in the pancreas of type 2 diabetic rat model and effects of rosiglitazone （RGZ） on it. Methods Type 2 diabetic rat model was induced by long-term feeding with high-fat foods followed by a single intraperitoneal injection of low dose of streptozotocin （STZ）. The morphological changes of pancreas were examined by microscopy and immunobistocbemistry. The mRNA levels of PDX-1 and insulin were determined by RT-PCR. PDX-1 protein expression was detected with Western blot. Results The percentage of insulin-positive cells（12. 75±2. 18） ,the average optical densities of PDX-1-positive area（0. 240 ± 0. 051）, PDX-1 mRNA level （0. 153 ± 0. 071） and PDX-1 protein level （0. 253 ± 0. 028） were significantly lower in untreated diabetic rats than in the controls （42. 61 ± 2.68,0. 648 ± 0. 087,0. 49 ± 0. 032, 0. 720 ± 0. 036 respectively）（all P〈0. 01）, and in RGZ-treated versus untreated group those parameters increased significantly.（all P〈0. 05 - 0. 01 ）. Conclusions Rosiglitazone could protect islet cell function, and improve PDX-1 expressions in both mRNA and protein levels.